Summary
A proteoglycan-specific antiserum has been used to monitor the effects of denervation in the electric organ of Torpedo marmorata. The antiserum was produced by injecting a highly purified synaptic vesicle fraction prepared from the electric organs of Torpedo marmorata. Following absorption the serum appears to be specific towards synaptic vesicles. The ultrastructural localization of the antigen determined by immuno-electron microscopy confirmed the specificity of the antiserum and showed that it did not crossreact with the proteoglycans of the basal lamina. The rate of disappearance of the vesicle proteoglycans following denervation was evaluated by means of the antiserum and was compared to the rate of disappearance of other vesicular and nerve terminal-associated markers. The results suggest that degeneration affects the vesicular constituents at varying rates resulting in a progressive disappearance of the entire functional capacity of the synaptic vesicles.
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References
Anderson MJ, Fambrough DM (1983) Aggregates of acetylcholine receptors are associated with plaques of a basal lamina heparan sulfate proteoglycan on the surface of skeletal muscle fibers. J Cell Biol 97:1396–1411
Boyne AF, Phillips TE (1983) Quick freezing preserves a large unstable vesicle population in torpidine electric organ nerve terminals. Abstr. Conference on dynamics of cholinergic function. Wilson Lodge at Oglebay Park, West Virginia, Oct. 31–Nov. 4
Ferretti P, Borroni E (1984) Effect of denervation on a cholinergic-specific ganglioside antigen (Chol-1) present in Torpedo electromotor presynaptic plasma membranes. J Neurochem 42:1085–1093
Fonnum F (1975) A rapid radiochemical method for the determination of choline acetyltransferase. J Neurochem 24:407–409
Fox GQ, Kötting D (1984) Torpedo electromotor system development: A quantitative analysis of synaptogenesis. J Comp Neurol 224:337–343
Jones RT, Walker JH, Stadler H, Whittaker VP (1982) Immunohistochemical localization of a synaptic vesicle antigen in a cholinergic neurone under conditions of stimulation and rest. Cell Tissue Res 223:117–126
Jones RT, Walker JH, Stadler H, Whittaker VP (1982) Further evidence that glycosaminoglycan specific to cholinergic synaptic vesicles recycles during electrical stimulation of the electric organ in Torpedo marmorata. Cell Tissue Res 224:685–688
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophagae T4. Nature 227:680–685
McKay R, Raff MC, Reichardt LF (eds) (1981) Monoclonal antibodies to neural antigens. Cold Spring Harbor Reports in Neurosciences, Vol. 2. Cold Spring Harbor Laboratory
Stadler H, Dowe GHC (1982) Identification of a heparan sulphatecontaining proteoglycan as a specific core component of cholinergic vesicles from Torpedo marmorata. EMBO J 1:1381–1384
Stanley PE, Williams SG (1969) Use of liquid scintillation spectrometry for determining adenosine triphosphate by the luciferase enzyme. Anal Biochem 29:381–392
Tashiro T, Stadler H (1978) Chemical composition of cholinergic synaptic vesicles from Torpedo marmorata based on improved purification. Eur J Biochem 90:479–487
Walker JH, Jones RT, Obrocki J, Richardson GP, Stadler H (1982) Presynaptic plasma membrane and synaptic vesicles of cholinergic nerve endings demonstrated by means of specific antisera. Cell Tissue Res 223:101–116
Walker JH, Obrocki J, Zimmermann CW (1983) Identification of a proteoglycan antigen characteristic of cholinergic synaptic vesicles. J Neurochem 41:209–216
Whittaker VP, Barker LA (1972) The subcellular fractionation of brain tissue with special reference to the preparation of synaptosomes and their component organelles. In: Fried R (ed) Methods in neurochemistry, Marcel Dekker, New York. Vol 2, pp 152
Whittaker VP, Essman WB, Dowe GHC (1972) The isolation of pure cholinergic synaptic vesicles from the electric organs of elasmobranch fish of the family Torpedinidae. Biochem J 128:833–846
Zimmermann H (1979) Vesicle recycling and transmitter release. Neuroscience 4:1773–1802
Zimmermann H, Whittaker VP (1974) Effect of electrical stimulation on the yield and composition of synaptic vesicles from the cholinergic synapses of the electric organ of Torpedo: a combined biochemical, electrophysiological and morphological study. J Neurochem 27:435–450
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Borroni, E., Ferretti, P., Fiedler, W. et al. The localization and rate of disappearance of a synaptic vesicle antigen following denervation. Cell Tissue Res. 241, 367–372 (1985). https://doi.org/10.1007/BF00217182
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DOI: https://doi.org/10.1007/BF00217182