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Histochemistry of sarcoplasmic reticulum Ca-ATPase using dysprosium as capturing reagent

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Summary

This report describes the development of a histochemical method for the demonstration of sarcoplasmic reticulum Ca-ATPase activity in cross-sections of skeletal muscle. The demonstration of sarcoplasmic reticulum Ca-ATPase activity is complicated by the fact that capturing reagents for phosphate inhibit the enzyme. We present a minimal model for heavy-metal-phosphate precipitation reactions which gives a theoretical description of the effect of enzyme inhibition on the rate of phosphate precipitation in the section. The model indicates that the choice of capturing reagent is crucial: whether or not ATPase activity can be demonstrated depends mainly on the inhibition constant and the solubility product of the phosphate salt of the capturing reagent (but also on a fairly large number of other factors). All lanthanides tested can be used to demonstrate sarcoplasmic reticulum Ca-ATPase activity, but dysprosium results in the highest staining intensity. This suggests that dysprosium inhibits sarcoplasmic reticulum Ca-ATPase to a lesser degree than the other lanthanides and/or the solubility product of its phosphate salt is smaller. As an example, the method is used to investigate the effect of thyroid hormone on sarcoplasmic reticulum Ca-ATPase activity in individual fibres of the rat soleus muscle.

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Authors and Affiliations

  1. Laboratory for Physiology, Institute for Cardiovascular Research (ICaR-VU), Vrije Universiteit, Van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands

    W. J. Van Der Laarse, P. Van Noort, W. S. Simonides, M. B. E. Lee-De Groot & C. Van Hardeveld

  2. Department of Experimental Zoology, Universiteit van Amsterdam, Kruislaan 320, 1098 SM, Amsterdam, The Netherlands

    P. C. Diegenbach

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  1. W. J. Van Der Laarse
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  2. P. Van Noort
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  3. W. S. Simonides
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  4. P. C. Diegenbach
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  5. M. B. E. Lee-De Groot
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  6. C. Van Hardeveld
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Van Der Laarse, W.J., Van Noort, P., Simonides, W.S. et al. Histochemistry of sarcoplasmic reticulum Ca-ATPase using dysprosium as capturing reagent. Histochem J 27, 702–714 (1995). https://doi.org/10.1007/BF00216684

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  • DOI: https://doi.org/10.1007/BF00216684

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