Summary
In three species of teleosts — carp Cyprinus carpio; grass carp Ctenopharyngodon idella; and crucian carp Carassius auratus — the caudal neurosecretory system displays small, medium-sized and large neurons. Urotensin I (UI)-immunoreactive and UI-nonreactive neurons were found in all three groups; in general, the number of the latter neurons exceeded that of the former. Noteworthy are: (i) UI-immunoreactive fibers in the caudal spinal cord and (ii) dense accumulations of UI-immunoreactive product around the capillaries of the urophysis. In two species of elasmobranchs — cat shark Heterodontus japonicas and swell shark Cephaloscyllium umbratile — neurosecretory neurons decreased in size in rostro-caudal direction. Most of the neurosecretory perikarya, their axons and the corresponding neurohemal areas were UI-immunoreactive, but a small number of secretory neurons was devoid of immunoreaction. Oxytocin, arginine vasopressin, substance P, somatostatin, neurotensin, vasoactive intestinal polypeptide and gastrin-releasing peptide were not detected in the caudal neurosecretory system of the carp.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bern HA, Pearson D, Larson BA, Nishioka RS (1985) Neurohormone from fish tails — the caudal neurosecretory system I. “Urophysiology” and the caudal neurosecretory system of fishes. Recent Prog Hormone Res 41:533–552
Fisher AWF, Wong K, Gill V, Lederis K (1984) Immunocytochemical localization of urotensin I in the caudal neurosecretory system of the white sucker (Catostomus commersoni). Cell Tissue Res 235:19–23
Lacanilao F (1972) The urophysial hydrosmotic factor of fishes. II. Chromatographic and pharmacologic indications of similarity to arginine vasotocin. Gen Comp Endocrinol 19:413–420
Larson BA, Nishioka RS, Pearson D, Bern HA (1984) Urotensins I and II: Presence in the same or different neurons of a fish caudal neurosecretory system? Am Zool 24:54A
Onstott D, Elde R (1984) Immunohistochemical localization of urotensin I/corticotropin-releasing factor immunoreactivity in neurosecretory neurons in the caudal spinal cord of fish. Neuroendocrinology 39:503–509
Osakada F, Ishii Y, Ichikawa T, Lederis K, Ohashi S, Shiraki M, Munekata E (1984) Synthesis of carp-urotensin I. In: Munekata E (ed) Peptide Chemistry 1983. Protein Research Foundation, Osaka, pp 267–272
Owada K, Kawata M, Akaji K, Takagi A, Moriga M, Kobayashi H (1985a) Urotensin II-immunoreactive neurons in the caudal neurosecretory system of freshwater and seawater fish. Cell Tissue Res 239:349–354
Owada K, Yamada C, Kobayashi H (1985b) Immunohistochemical investigation of urotensins in the caudal spinal cord of elasmobranchs and the lamprey, Lampetra japonica. Cell Tissue Res 242:527–530
Renda T, D'Este L, Negri L, Lomoanto D (1982) Sauvagine-like immunoreactivity in the bony fish urophysis and caudal neurosecretory system. Basic Appl Histochem 26:89–98
Sternberger LA, Hardy PH, Cuclis JJ Jr, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti-horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Yamada C, Owada K, Kobayashi H (1985) Colocalization of corticotropin-releasing factor/urotensin I and urotensin II in the caudal neurosecretory neurons in the carp, Cyprinus carpio. Zool Sci 2:813–816
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamada, C., Yamada, S., Ichikawa, T. et al. Immunohistochemical localization of urotensin I and other neuropeptides in the caudal neurosecretory system of three species of teleosts and two species of elasmobranchs. Cell Tissue Res. 244, 687–690 (1986). https://doi.org/10.1007/BF00212550
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00212550