Summary
Uropepsinogen (PGA) was isolated and purified from human urine using a column chromatography series. The purified PGA was injected into a rabbit and a PGA-specific antibody was obtained. PGA isozymogen in human urine could be detected reproducibly by immunoblotting using this antibody after isoelectric focusing electrophoresis (IEF) on polyacrylamide gels. This technique may prove to be useful in the genetic study of PGA polymorphism.
Zusammenfassung
Uropepsinogen (PGA) wurde aus Humanharn mit Hilfe einiger Säulenchromatographieverfahren isoliert und gereinigt. Das gereinigte PGA wurde ins Kaninchen eingespritzt und dadurch ein spezifischer Antikörper gegen PGA gewonnen. PGA-Isozymogen im Harn ließ sich durch Einführung der kombinierten Technik des Anti-PGA-benutzten Immunoblotts und isoelektrischer Fokussierung (IEF) auf Polyacrylamidgelen darstellen. Die Anwendung dieser Technik kann sich bei genetischen Untersuchungen des PGA als nützlich erweisen.
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Supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan
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Yasuda, T., Ikehara, Y., Sato, W. et al. New detection method for uropepsinogen (PGA) using isoelectric focusing and immunoblotting techniques. Z Rechtsmed 98, 147–153 (1987). https://doi.org/10.1007/BF00200337
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DOI: https://doi.org/10.1007/BF00200337