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An inositol 1,4,5-trisphosphate-6-kinase activity in pea roots

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Abstract

A soluble extract from pea (Pisum sativum L.) roots, when incubated with ATP and inositol 1,4,5-trisphosphate, produced an inositol tetrakisphosphate. The chromatographic properties of this inositol tetrakisphosphate, and of the products formed by its chemical degradation, identify it as inositol 1,4,5,6-tetrakisphosphate. No evidence was obtained for a 3-phosphorylation of inositol 1,4,5-trisphosphate. The importance of these observations with respect to inositol phosphates and calcium signalling in higher plants, is discussed.

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Abbreviations

HPLC:

high-performance liquid chromatography

Ins(1,4,5)P3 :

inositol 1,4,5-trisphosphate

InsP4 :

inositol tetrakisphosphate

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Authors and Affiliations

  1. Department of Cell Biology, John Innes Institute, Colney Lane, NR4 7UH, Norwich, Norfolk, UK

    Jayne A. Chattaway, Bjørn K. Drøbak & Peter A. C. Watkins

  2. School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, Norfolk, UK

    Alan P. Dawson

  3. Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, CB2 4AT, Babraham, Cambridge, UK

    Andrew J. Letcher, Leonard R. Stephens & Robin F. Irvine

Authors
  1. Jayne A. Chattaway
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  2. Bjørn K. Drøbak
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  3. Peter A. C. Watkins
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  4. Alan P. Dawson
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  5. Andrew J. Letcher
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  6. Leonard R. Stephens
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  7. Robin F. Irvine
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Additional information

J.A.C. gratefully acknowledges support from the Agricultural and Food Research Council, U.K., Plant Molecular Biology Initiative.

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Chattaway, J.A., Drøbak, B.K., Watkins, P.A.C. et al. An inositol 1,4,5-trisphosphate-6-kinase activity in pea roots. Planta 187, 542–545 (1992). https://doi.org/10.1007/BF00199975

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  • DOI: https://doi.org/10.1007/BF00199975

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