Abstract
Stem node culture of the potato (Solanum tuberosum) cv. Sante was used to examine the phenotypical alterations due to different levels of endogenous cytokinins. The altered phenotype, which dramatically deviates from the control phenotype, was induced after treatment of plantlets with 1 μm jasmonic acid. Plantlets grown on the medium supplemented with jasmonic acid were taller, with well developed root systems, expanded leaves, thickened stems, and they showed hyperhydric symptoms. Their cytokinin content was about half that of the control plantlets. Morphologic characteristics corresponding to transgenic plants that overproduce cytokinins, including release of axillary buds and inhibited rooting, correlated with the high cytokinin levels in control plants.
Similar content being viewed by others
Abbreviations
- JA:
-
jasmonic acid
- Z:
-
trans-zeatin
- ZR:
-
trans-zeatin riboside
- ZRMP:
-
zeatin riboside 5′-monophosphate
- Z-9-G:
-
trans-zeatin N-9-glucoside
- DHZ:
-
dihydrozeatin
- DHZR:
-
dihydrozeatin riboside
- DHZRMP:
-
dihydrozeatin riboside 5′-monophosphate
- DHZ-9-G:
-
dihydrozeatin 9-glucoside
- iP:
-
iso-pentenyladenine
- iPA:
-
iso-pentenyladenosine
- iP-9-G:
-
iso-pentenyladenine 9-glucoside
- HPLC:
-
high performance liquid chromatography
References
Biddington NL, Thomas TH (1973) A modified Amaranthus betacyanin bioassay for the rapid determination of cytokinin in plant extracts. Planta 111:183–186
Binns AN (1994) Cytokinin accumulation and action: Biochemical, genetic and molecular approaches. Annu Rev Plant Physiol Plant Mol Biol 45:173–196
Brzobohatý B, Moore I, Palme K (1994) Cytokinin metabolism: Implications for regulation of plant growth and development. Plant Mol Biol 26:1483–1497
Debergh P, Aitken-Christie J, Cohen D, Grout B, von Arnold S, Zimmerman R, Ziv M (1992) Reconsideration of the term “vitrification” as used in micropropagation. Plant Cell Tiss Org 30:135–140
Demmig-Adams B, Adams WW III (1996) The role of xanthophyll cycle carotenoids in the protection of photosynthesis. Trends Plant Sci 1:21–26
Dermastia M, Kovač M, Ravnikar M (1994a) Cytokinin level in healthy and systemically potato virus M-infected potato (Solanum tuberosum cv. Vesna). In: Abstracts of Fourth International Congress of Plant Molecular Biology, Amsterdam 1994, abstract 1508
Dermastia M, Ravnikar M (1996) Altered cytokinin pattern and enhanced tolerance to potato virus YNTN in the susceptible potato cultivar (Solarium tuberosum cv. Igor) grown in vitro. Physiol Mol Plant Pathol 47, 48:65–71
Dermastia M, Ravnikar M, Kovač M (1995) Increased cytokinin-9-glucosilation in roots of susceptible Solanum tuberosum cultivar infected by potato virus YNTN. Mol Plant Micr Inter 8:327–330
Dermastia M, Ravnikar M, Vilhar B, Kovač M (1994b) Increased level of cytokinin ribosides in jasmonic acid-treated potato (Solanum tuberosum) stem node cultures. Physiol Plant 92:241–246
Koda Y (1992) The role of jasmonic acid and related compounds in the regulation of plant development. Int Rev Cytol 135:155–199
Kovač M, Ravnikar M (1994) The effect of jasmonic acid on the photosynthetic pigments of potato plants in vitro. Plant Sci 103:11–17
Kovač M, Žel J (1994) The effect of aluminium on the cytokinins in the mycelia of Lactarius piperatus. Plant Sci 97:137–142
Kovač M, Žel J (1995) The effect of aluminium on cytokinins in the mycelia of Amanita muscaria. J Plant Growth Regul 14:117–120
Kusnetsov VV, Oelmüller R, Sarwat MI, Porfirova SA, Cherepneva GN, Herrmann RG, Kulaeva ON (1994) Cytokinins, abscisic acid and light affect accumulation of chloroplast proteins in Lupinus luteus cotyledons without notable effect on steady-state mRNA levels. Planta 194:318–327
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nicander B, Stähl U, Björkman P, Tillberg E (1993) Immunoaffinity co-purification of cytokinins and analysis by HPLC with ultraviolet-spectrum detection. Planta 189:312–320
Singh S, Letham DS, Palni LMS (1992) Effect of growth substances and mineral nutrients on cytokinin levels and senescence of tobacco (Nicotiana rustica) leaves. In: Karssen CM, van Loon LC, Vreugdenhil D (eds) Progress in plant growth regulation. Kluwer Academic Publishers, Netherlands, pp 628–634
Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol 11:118–130
Turnbull CGN, Hanke DE (1985) The control of bud dormancy in potato tubers: Measurement of the seasonal pattern of changing concentration of zeatin-cytokinins. Planta 165:366–376
Yusibov VM, Il PC, Andrianov VM, Piruzian ES (1991) Phenotypically normal transgenic T-cyt tobacco plants as a model for the investigation of plant gene expression in response to phytohormonal stress. Plant Mol Biol 17:825–836
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dermastia, M., Ravnikar, M. & Kovač, M. Morphology of potato (Solanum tuberosum L. cv. Sante) stem node cultures in relation to the level of endogenous cytokinins. J Plant Growth Regul 15, 105–108 (1996). https://doi.org/10.1007/BF00198923
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00198923