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The effect of aluminum on cytokinins in the mycelia of Amanita muscaria

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Abstract

High performance liquid chromatography analysis of immunoaffinity-purified extracts of mycelia of Amanita muscaria, and the Amaranthus bioassay of the eluted fractions, revealed the following seven cytokinins: zeatin, zeatin riboside, zeatin N-9-glucoside, dihydrozeatin, dihydrozeatin riboside, isopentenyl adenine, and isopentenyl adenosine. The decreased growth of aluminum-treated mycelia correlated with a 35% decrease in the total amount of the cytokinins. Among individual cytokinins, zeatin was the most affected, exhibiting a reduction of about 90%. The results are compared with previous investigations of aluminum effects on cytokinins in the mycelia of Lactarius piperatus, whose growth is stimulated by aluminum.

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Abbreviations

ZR:

zeatin riboside

iPA:

isopentenyl adenosine

Z:

zeatin

DHZ:

dihydrozeatin

iP:

isopentenyl adenine

DHZR:

dihydrozeatin riboside

Z-9G:

zeatin N-9-glucoside

iP-9G:

isopentenyl N-9-glucoside

HPLC:

high performance liquid chromatography

DHZRMP:

dihydrozeatin riboside monophosphate

ZRMP:

zeatin riboside monophosphate

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Authors and Affiliations

  1. National Institute of Biology, Karlovška 19, 61000, Ljubljana, Slovenia

    Maja Kovač & Jana Žel

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  1. Maja Kovač
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  2. Jana Žel
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Kovač, M., Žel, J. The effect of aluminum on cytokinins in the mycelia of Amanita muscaria . J Plant Growth Regul 14, 117–120 (1995). https://doi.org/10.1007/BF00210912

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  • DOI: https://doi.org/10.1007/BF00210912

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