Summary
The channel properties of nicotinic acetylcholine receptor subtypes in the nervous system of insects (Locusta migratoria) have been characterized. Single channel measurements were performed using patchclamp techniques as well as planar lipid bilayer reconstitution approaches. In reconstitution experiments using receptor-preparations isolated from neuronal membranes by α-toxin affinity chromatography, a ligand-gated channel type was found, which showed a high conductance and a short mean lifetime. Patch-clamp experiments on synapse-free somata of isolated nerve cells revealed an acetylcholine-gated channel type with a lower conductance but a longer lifetime. The two different agonist-activated channel types are supposed to represent synaptic and extrasynaptic acetylcholine receptors.
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Tareilus, E., Hanke, W. & Breer, H. Neuronal acetylcholine receptor channels from insects: a comparative electrophysiological study. J Comp Physiol A 167, 521–526 (1990). https://doi.org/10.1007/BF00190822
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DOI: https://doi.org/10.1007/BF00190822