Skip to main content
SpringerLink
Log in

The relationship between HTLV-I-infected cell lines and uveitis

  • Laboratory Investigation
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

• Background: Recently it has been revealed that human T-lymphotropic virus type I (HTLV-I) infection causes uveitis in human. We previously reported HTLV-I uveitis in a rabbit. To investigate the relationship between HTLV-I infection and uveitis, we established an HTLV-I-infected T-cell clone from the cells infiltrated in the anterior chamber of this rabbit and compared the viral production with that in other HTLV-I-infected cell lines. • Methods: The clonality was determined by Southern blot hybridization with various restriction enzymes. Flow-cytometric analysis was used for investigating the expression of cell surface antigens. To compare viral production, we performed reverse transcriptase assay of the culture media and inhibition enzyme-linked immunosorbent assay to determine the quantity of intracellular HTLV-I antigens. • Results: The established clone was Ia (MHC class II) positive T cell. This T-cell clone was able to produce about three times more HTLV-I antigens than other HTLV-I-infected cell lines tested. • Conclusion: A T-cell clone established from anterior aqueous of an HTLV-I uveitis rabbit can produce more HTLV-I antigen than other HTLV-I-infected cell lines tested and it can be recognized easily by the immune system. Therefore, this high virus production may have a causal relation to uveitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Poiesz BJ, Ruscetti FW, Gazdar AF, et al. (1980) Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci USA 77:7415–7419

    Google Scholar 

  2. Rodgers-Johnson P, Gajdusek DC, Morgan OSC, et al. (1985) HTLV-I and HTLV-III antibodies and tropical spastic paraparesis. Lancet ii:1247–1248

    Google Scholar 

  3. Nishioka K, Maruyama I, Sato K, et al. (1991) Chronic inflammatory arthropathy associated with HTLV-I. Lancet 1:441

    Google Scholar 

  4. Mochizuki M, Watanabe T, Yamaguchi K, et al. (1992) HTLV-I uveitis: a distinct clinical entity caused by HTLV-I. Jpn J Cancer Res 83:236–239

    Google Scholar 

  5. Suciu-Foca N, Rubinstein P, Popovic M, et al. (1984) Reactivity of HTLV-transformed human T-cell lines to MHC class 11 antigens. Nature 312:275–277

    Google Scholar 

  6. Suciu-Foca N, Rubinstein P, Rohowsky-Kochan C, et al. (1986) Functional modifications of alloreactive T cell clones infected with HTLV-I. J Immunol 137:1115–1119

    Google Scholar 

  7. Sugamura K, Matuyama M, Fujii M, et al. (1983) Establishment of human cell lines constitutively producing immune interferon: transformation of normal T-cells by a human retrovirus. J Immunol 131:1611–1612

    Google Scholar 

  8. Shimizu K, Hirano T, Ishibashi K, et al. (1985) Immortalization of BGDF (BCGF II) and BCDF-producing T cells by human T cell leukemia virus (HTLV) and characterization of human BCDF (BCGF II). J Immunol 134:1728–1733

    Google Scholar 

  9. Ishiguro N, Abe N, Seto K, et al. (1992) A rat model of human T lymphocyte virus type I (HTLV-I) infection. 1. Humoral antibody response, provirus integration, and HTLV-I-associated myelopathy/tropical spastic paraparesis-like myelopathy in seronegative HTLV-I carrier rats. J Exp Med 176:981–989

    Google Scholar 

  10. Iwakura T, Tosu M, Yoshida E, et al. (1991) Induction of inflammatory arthropathy resembling rheumatoid arthritis in mice transgenic for HTLV-I. Science 253:1026–1028

    Google Scholar 

  11. Taguchi H, Sawada T, Fukushima A et al. (1993) Bilateral uveitis in a rabbit experimentally infected with human T lymphotropic virus type I. Lab Invest 69:336–339

    Google Scholar 

  12. Fukushima A, Ueno H, Fujimoto S (1994) Antigenic cross-reactivity between human T lymphotropic virus type I (HTLV-I) and retinal antigens recognized by T cells. Clin Exp Immunol 95:459–464

    Google Scholar 

  13. Miyoshi I, Kubonishi I, Yoshimoto S, et al. (1981) Type C virus particles in a cord T-cell line derived by co-cultivation of normal human cord leukocytes and human leukaemic T-cells. Nature 294:770–771

    Google Scholar 

  14. Miyoshi I, Yoshimoto S, Kubonishi I, et al. (1985) Infectious transmission of human T-cell leukemia virus to rabbits. Int J Cancer 35:81–85

    Google Scholar 

  15. Tanaka Y, Iwahara Y, Sawada T et al. (1992) Cross-neutralization between prototype Japanese HTLV-I and a Melanesian variant of HTLV-I. Proceedings of the Japanese Cancer Association, 51st Annual Meeting, Osaka, September, p 79

  16. Hirose S, McAllister CG, Mittal KK, et al. (1988) A cell line and clones of lymphocytes from a healthy donor, with specificity to S-antigen. Invest Ophthalmol Vis Sci 29:1636–1641

    Google Scholar 

  17. Jackson S, Chused TM, Wilkinson JM, et al. (1983) Differentiation antigens identify subpopulations of rabbit T and B lymphocytes: definition by flow cytometry. J Exp Med 157:34–46

    Google Scholar 

  18. Lobel SA, Knight KL (1984) The role of Ia molecules in immune functions as determined with the use of an antila monoclonal antibody. Immunology 51:35–43

    Google Scholar 

  19. Kataoka R, Takehara N, Iwahara Y, et al. (1991) Transmission of HTLV-I by blood transfusion and its prevention by passive immunization in rabbits. Blood 76:1657–1661

    Google Scholar 

  20. Rho HM, Poiesz B, Ruscetti FW, et al. (1981) Characterization of the reverse transcriptase from a new retrovirus (HTLV) produced by a human cutaneous T-cell lymphoma cell line. Virology 112:355–360

    Google Scholar 

  21. Yoshida M, Seiki M, Yamaguchi K, et al. (1984) Monoclonal integration of human T-cell leukemia provirus in all primary tumors of adult T-cell leukemia suggests causative role of human T-cell leukemia virus in the disease. Proc Natl Acad Sci USA 81:2534–2537

    Google Scholar 

  22. Mochizuki M, Kuwabara T, McAllister C, et al. (1985) Adoptive transfer of experimental autoimmune uveoretinitis in rats. Invest Ophthalmol Vis Sci 26:1–9

    Google Scholar 

  23. Caspi RR, Roberge FG, McAllister C, et al. (1986) T cell lines mediating experimental autoimmune uveoretinitis (EAU) in the rat. J Immunol 136:928–936

    Google Scholar 

  24. Mittler RS, Rao PE, Talle MA, et al. (1983) Cell membrane perturbation of resting T cells and thymocytes causes display of activation antigens. J Exp Med 158:99–111

    Google Scholar 

  25. Salahuddin SZ, Markham PD, Lindner SG, et al. (1984) Lymphokine production by cultured human T cells transformed by human T-cell leukemia-lymphoma virus type I. Science 223:703–707

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Kochi Medical School, Kohasu, Oko-cho, 783, Nankoku, Japan

    Atsuki Fukushima & Hisayuki Ueno

Authors
  1. Atsuki Fukushima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hisayuki Ueno
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fukushima, A., Ueno, H. The relationship between HTLV-I-infected cell lines and uveitis. Graefe's Arch Clin Exp Ophthalmol 233, 231–235 (1995). https://doi.org/10.1007/BF00183597

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00183597

Keywords

Search

Navigation