Summary
A new method, which employs a sequence of heteronuclear-homonuclear-heteronuclear Hartmann-Hahn (HEHOHEHAHA) cross-polarization steps for obtaining through-bond H-C-C-H correlations in larger proteins (Mr > 15 kDa), is presented. The method has significantly higher sensitivity compared to INEPTHOHAHA-INEPT-based techniques. An additional feature of this experiment is that well-phaseable spectra may be obtained with a minimal (4-step) phase cycle and, consequently, experimental time can be utilized towards obtaining high resolution in indirect dimensions. Results from 2D and 3D HEHOHEHAHA experiments on T4-lysozyme are presented.
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Majumdar, A., Wang, H., Morshauser, R.C. et al. Sensitivity improvement in 2D and 3D HCCH spectroscopy using heteronuclear cross-polarization. J Biomol NMR 3, 387–397 (1993). https://doi.org/10.1007/BF00176006
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DOI: https://doi.org/10.1007/BF00176006