Summary
Cations were precipitated with potassiumpyroantimonate in the middle ear mucosa of the rat and the distribution of the formed precipitates was studied by electron microscopy. The precipitate density in various cells of the different epithelia of the middle ear mucosa was determined on electron micrographs by counting the number of precipitates per unit area. Electron spectroscopic imaging was also performed to obtain information about the spatial distribution of the precipitates and their elemental composition.
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References
Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (1983) Molecular biology of the cell. Garland, New York
Albiin N, Hellstrom S, Stenfors L-A, Cerne A (1986) Middle ear mucosa in rats and humans. Ann Otol Rhinol Laryngol 95 [Suppl 126]:2–15
Ando T, Mayahara H, Fujimoto K, Miyajima H, Ogawa K (1982) Ultracytochemical localization of Ca2+-ATPase activity on the epithelial cells of rat trachea. Acta Histochem Cytochem 15:812–826
Bessen M, Fay RB, Witman GB (1980) Calcium control of waveform in isolated flagellar axonemes of chlamydomonas. J Cell Biol 86:446–455
Bortnick E, Proud GO (1965) Experimental absorption of fluids from the middle ear. Arch Otolaryngol 81:237–242
Bulger RE (1969) Use of potassium pyroantimonate in the localization of sodium ions in rat kidney tissue. J Cell Biol 40:79–94
Campbell AK (1983) Intracellular calcium: it's universal role as regulator. Wiley, New York
Clemente F, Meldolesi J (1975) Calcium and pancreatic secretion. 1. Subcellular distribution of calcium and magnesium in the exocrine pancreas of the guinea pig. J Cell Biol 65:88–102
Davis WL, Jones RG, Hagler HK (1979) Calcium containing lysosomes in the normal chick duodenum: a histochemical and analytical electron microscopic study. Tissue Cell 11:127–138
Heinrich U-R, Mann W (1988) Ultrastructural localization of Cat+-binding sites in the spiral limbus, the stria vascularis and Reissner's membrane of the guinea pig. Arch Otorhinolaryngol 245:279–283
Herman L, Sato T, Hales CN (1973) The electron microscopic localization of cations to pancreatic islets of Langenhans and their possible role in insulin secretion. J Ultrastruct Res 42:298–311
Leuschen MP, Moriarty CM, Sampson HW, Piscopo I (1981) Cytochemical analysis of intracellular calcium distribution in the anterior pituitary of the rat. Cell Tissue Res 220:191–200
Lim DJ (1974) Functional morphology of the lining membrane of the middle ear and eustachian tube. Ann Otol Rhinol Laryngo 183 Suppl 11:5–26
Lim DJ, De Maria TF, Bakaletz LO (1987) Functional morphology of the tubotympanum related to otitis media. Adv Otorhinolaryngol 37:21–26
Mann W (1986) Experimental tympanosclerosis following infection with streptococcus pyogenes and vitamin D3 intoxication. Arch Otorhinolaryngol 243:296–303
Mann W, Heinrich U-R (1988) Cation distribution in the organ of Corti of the guinea pig. Arch Otorhinolaryngol 245:185–190
Mann W, Beck C, Schaefer HE (1987) The significance of calcium antagonists in rat experimental tympanoslcerosis. Arch Otorhinolaryngol l243:382–386
Oschman JL, Wall BJ (1972) Calcium binding to intestinal membranes. J Cell Biol 55:58–73
Ottensmeyer FP, Andrew JW (1980) High-resolution microanalysis of biological specimens by electron energy loss spectroscopy and by electron spectroscopic imaging. J Ultrastruct Res 72:336–348
Penniston JT (1983) Plasma membrane Ca2+-ATPase as active Ca2+ pumps. In: Cheung WY (ed) Calcium and cell function, vol. IV. Academic Press, New York, pp 99–149
Ravazzola M, Malaisse-Lagae F, Amherdt M, Perrelet A, Malaisse WJ, Orci L (1976) Patterns of calcium localization in pancreatic endocrine cells. J Cell Sci 27:107–117
Sadé J, Eliezer N, Silberberg A, Nevo AC (1970) The role of mucus in transport by cilia. Am Rev Respir Dis 102:48–52
Sato A, Spicer SS (1981) Subcellular distribution of ionic components in gastric mucosa of the guinea pig. Cell Tissue Res 219:143–158
Schafer H (1979) Zellcalcium and Zellfunktion. Fischer, Stuttgart
Simson JAV, Spicer SS (1975) Selective subcellular localization of cations with variants of the potassium (pyro)antimonate technique. J Histochem Cytochem 23:575–598
Spurr AR (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Terrahe K, Westphal U (1971) Elektronenmikroskopische Resorptionsstudien an der gesunden and entziindlichen Mittelohrschleimhaut. Arch Klin Exp Ohren Nasen Kehlkopfheilkd 199:458–462
Ueno S, Umar H, Bambauer HJ, Ueck M (1984) Ultracytochemical localization of Ca++-ATPase activity in the paraphyseal epithelial cells of the frog, Rana esculenta. Cell Tissue Res 235:3–11
Weakly BS (1979) A variant of the pyroantimonate technique suitable for localization of calcium in ovarian tissue. J Histochem Cytochem 27:1017–1028
Wick SM, Hepler PK (1982) Selective localization of intracellular Ca2+ with potassium antimonate. J Histochem Cytochem 30:1190–1204
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Karatay, M.C., Mann, W. & Heinrich, U.R. Ultrastructural localization of Ca2+-binding sites in the middle ear mucosa of the rat. Eur Arch Otorhinolaryngol 247, 156–160 (1990). https://doi.org/10.1007/BF00175968
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DOI: https://doi.org/10.1007/BF00175968