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Histochemical distribution of zinc in the brain of the rainbow trout, Oncorhynchos myciss

II. The diencephalon

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Summary

The distribution of zinc in the diencephalon of the rainbow trout, Oncorhynchos myciss, is described in the present paper, which is the second in a series of three reporting for the first time the distribution of a heavy metal in the fish brain. The Neo-Timm method was used for the histochemical demonstration of zinc. The staining was essentially confined to the neuropil, in all probability representing stained axon terminals, but stained nerve cell bodies were observed in the nucleus lateralis geniculatus and the nucleus cerebellosus hypothalami. Stained fibers were never seen. The staining gave rise to a consistent, specific distribution pattern, which accorded well with the diencephalic nuclei defined on the basis of cytoarchitectural criteria. The diencephalon was in general stained with much higher intensity than the telencephalon, in surprising contrast to the state of affairs in the mammalian, reptilian, and avian brain. In species of these classes, high staining intensities are observed almost exclusively in the telencephalon. The Neo-Timm staining was predominantly distributed in the nuclei of the periventricular zone, but some internal (migrated) nuclei did show a positive staining reaction, namely the nucleus lateralis geniculatus, the anterior thaamic nucleus, the nucleus diffusus tori lateralis, and the nucleus cerebellosus hypothalami. The zinc distribution pattern has been compared with the terminal fields of afferent projections, known from experimental studies, and with the distribution of substance P. The possible function of zinc in synaptic vesicles is considered.

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Authors and Affiliations

  1. Department of Neurobiology, Institute of Anatomy, University of Aarhus, DK-8000, Aarhus C, Denmark

    Carmen Piñuela & Finn A. Geneser

  2. Department of Zoology, Institute of Biology, University of Aarhus, DK-8000, Aarhus C, Denmark

    Erik Baatrup

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  1. Carmen Piñuela
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  2. Erik Baatrup
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  3. Finn A. Geneser
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Piñuela, C., Baatrup, E. & Geneser, F.A. Histochemical distribution of zinc in the brain of the rainbow trout, Oncorhynchos myciss . Anat Embryol 186, 275–284 (1992). https://doi.org/10.1007/BF00174150

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  • DOI: https://doi.org/10.1007/BF00174150

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