Summary
The effects of acute continuous exposure to the smoke from 1–4 cigarettes have been studied in the male rat in terms of hypothalamic catecholamine levels and utilization as well as the secretion of anterior pituitary hormones. Catecholamine levels in discrete hypothalamic catecholamine nerve terminal systems were studied by quantitative histofluorimetry. Catecholamine utilization was studied by means of the tyrosine hydroxylase inhibition method using α-methyl-(±)-p-tyrosine methyl ester. The serum hormone levels of adenohypophyseal hormones and of corticosterone were measured by the use of radioimmunoassay procedures. The results show that acute continuous exposure to unfiltered but not to filtered (Cambridge glass fibre filters) cigarette smoke leads to small but dose-dependent reductions of amine levels in most of the hypothalamic noradrenaline and dopamine nerve terminal system. These effects were associated with an enhancement of regional hypothalamic noradrenaline utilization but not of dopamine utilization in the median eminence. Furthermore, a reduction of TSH and prolactin serum levels was noted as well as increases in ACTH secretion. These results are partly different from those previously obtained with rats acutely exposed to intermittent unfiltered cigarrete smoke. This difference is suggested to be due to a temporary blockade of catecholamine release following acute continuous exposure to cigarette smoke.
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Andén N-E, Corrodi H, Fuxe K (1969) Turnover studies using synthesis inhibition. In: Hooper G (ed) Metabolism of amines in the brain. Macmillan, London, pp 38–47
Agnati LF, Andersson K, Wiesel F, Fuxe K (1979) A method to determine dopamine levels and turnover rate in discrete dopamine nerve terminal systems by quantitative use of dopamine fluorescence obtained by Falck-Hillarp methodology. J Neurosci Methods 1:365–373
Andersson K, Fuxe K, Eneroth P, Gustafsson J-Å, Agnati LF (1980) Mecamylamine induced blockade of nicotine induced inhibition of gonadotrophin and TSH secretion and of nicotine induced increases of catecholamine turnover in the rat hypothalamus. Acta Physiol Scand (Suppl 108) 479:27–29
Andersson K, Fuxe K, Eneroth P, Blake CA, Agnati LF, Gustafsson J-Å (1981a) Effects of androgenic and adrenocortical steroids on hypothalamic and preoptic catecholamine nerve terminals and on the secretion of anterior pituitary hormones. In: Fuxe K, Hökfelt T, Luft R (eds) Steroid hormone regulation of the brain. Pergamon Press, Oxford New York, pp 117–133
Andersson K, Fuxe K, Eneroth P, Nyberg F, Roos P (1981b) Rat prolactin and hypothalamic catecholamine nerve terminal systems. Evidence for rapid and discrete increases in dopamine and noradrenaline turnover in the hypophysectomized male rat. Eur J Pharmacol 76:261–265
Andersson K, Fuxe K, Eneroth P, Agnati LF (1982a) Effects of acute central and peripheral administration of nicotine on hypothalamic catecholamine nerve terminal systems and on the secretion of adenohypophyseal hormones in the male rat. Med Biol 60:98–111
Andersson K, Fuxe K, Eneroth P, Agnati LF (1982b) Involvement of cholinergic nicotine-like receptors as modulators of amine turnover in various types of hypothalamic dopamine and noradrenaline nerve terminal systems of prolactin, LH, FSH and TSH secretion in the castrated male rat. Acta Physiol Scand 116:41–50
Andersson K, Siegel R, Fuxe K, Eneroth P (1983) Intravenous injections of nicotine induce very rapid and discrete reductions of hypothalamic catecholamine levels associated with increases of ACTH, vasopressin and prolactin secretion Acta Physiol Scand 118:35–40
Andersson K, Agnati LF, Fuxe K, Eneroth P, Härfstrand A, Benfenati F (1984) Corticotrophin-releasing factor increases noradrenaline turnover in the median eminence and reduces noradrenaline turnover in the paraventricular region of the hypophysectomized male rat. Acta Physiol Scand 120:621–624
Andersson K, Eneroth P, Roos P (1985a) Effects of TRH and a rat TSH preparation on discrete hypothalamic and forebrain catecholamine nerve terminal networks in the hypophysectomized male rat. Eur J Pharmacol 111:295–307
Andersson K, Fuxe K, Agnati LF (1985b) Determinations of catecholamine half-lives and turnover rates in discrete catecholamine nerve terminal systems of the hypothalamus, the preoptic region and the forebrain by quantitative histofluorimetry. Acta Physiol Scand 123:411–426
Andersson K, Fuxe K, Eneroth P, Mascagni F, Agnati LF (1985c) Effects of acute intermittent exposure to cigarette smoke on catecholamine levels and turnover in various types of hypothalamic DA and NA nerve terminal systems as well as on the secretion of adenohyophyseal hormones and corticosterone. Acta Physiol Scand 124:277–285
Curvall M, Kazemi-Vala E, Enzell CR (1982) Simultaneous determination of nicotine and cotinine in plasma using capillary column gas chromatography with nitrogen-sensitive detection. J Chromat 232:283–293
Falck B, Hillarp N-Å, Thieme G, Torp A (1962) Fluorescence of catecholamine and related compounds condensed with formaldehyde. J Histochem Cytochem 10:348–354
Fuxe K, Agnati LF, Andersson K, Eneroth P, Härfstrand A, Goldstein M, Bernardi B, Vale W, Yu Z-Y, Gustaffson J-Å (1985) The external layer of the median eminence and the paraventricular hypothalamic nucleus represent two important levels of integration in the neuroendocrine regulation. Studies on peptide-catecholamine interactions give evidence for the existence of “medianosomes”. In: del Pozo E, Flückiger E (eds) Dopamine and neuroendocrine active substances. Academic Press, New York, pp 11–18
Guerin M, Stockely J, Higgins C, Moneyhun J, Holmberg G (1979) Inhalation bioassay chemistry — Walton Horizontal Smoking Machine for inhalation exposure of rodents to cigarette smoke. J Nat Cancer Inst 63:441–448
Hökfelt T, Fuxe K, Johansson O, Jeffcoate S, White N (1975) Distribution of thyrotropin-releasing hormone (TRH) in the central nervous system as revealed with immunohistochemistry. Eur J Pharmacol 34:389–392
Johansson O, Hökfelt T, Jeffcoate SL, White N, Spindel E (1983) Light and electron microscopic immunohistochemical studies on TRH in the central nervous system of the rat. In: Griffiths EC, Bennett GW (eds) Thyrotropin-releasing hormone. Raven Press, New York, pp 19–31
Langley JN, Dickinson WL (1889) On the local paralysis of peripheral ganglia, and on the connection of different classes of nerve fibres with them. Proc Roy Soc 46:423–431
Larsson C, Nordberg A (1985) Comparative analysis of nicotine-like ligand receptor interactions in rodent brain homogenate. J Neurochem 45:24–31
Legros JJ, Smitz S, Conte-Devolx B (1979) Influence of nicotine on neuropituitary function. In: Remond A, Izard C (eds) Electrophysiological effects of nicotine. Elsevier/North-Holland Biomedical Press, Amsterdam, pp 217–225
Löfström A, Jonsson G, Fuxe K (1976) Microfluorimetric quantitation of catecholamine fluorescence of rat median eminence. I. Aspects on the distribution of dopamine and noradrenaline nerve terminals. J Histochem Cytochem 24:415–426
Nilsen OG, Toftgård R, Eneroth P (1980) Effects of acrylonitrile on rat liver cytochrome P-450, benzo(a)pyrene metabolism and serum hormone levels. Toxicol Lett 6:399–404
Snedecor GW, Cochran WG (1980) Statistical methods, 7. edn. Iowa State University Press, Ames, Iowa, USA
Trendelenburg U (1967) Some aspects of the pharmacology of autonomic ganglion cells. Rev Physiol Biochem Pharmacol 59:1–85
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This work has been supported by a grant (1223) from the Council for Tobacco Research, New York, USA and by a grant from the Svenska Tobaks monopolet
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Andersson, K., Fuxe, K., Eneroth, P. et al. Effects of acute continuous exposure of the rat to cigarette smoke on amine levels and utilization in discrete hypothalamic catecholamine nerve terminal systems and on neuroendocrine function. Naunyn-Schmiedeberg's Arch Pharmacol 335, 521–528 (1987). https://doi.org/10.1007/BF00169118
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DOI: https://doi.org/10.1007/BF00169118