Abstract
The distribution of the cytoskeletal elements cytokeratin 18, vimentin, desmin, glial fibrillary acid protein (GFAP), and actin was investigated in different portions of the ciliary body of human and bovine eyes. Regional differences were found only for vimentin and cytokeratin. In both species, cytokeratin staining was more intense in the pigmented (PE) than in the nonpigmented epithelial (NPE) cells. In contrast, immunostaining for vimentin was more intense in the NPE than in the PE. The most intense immunoreactivity for cytokeratin 18 in the PE and for vimentin in the NPE was observed in the posterior pars plana and the crests of the pars plicata. In the remaining portions of the ciliary body, staining was reduced or absent. Desmin and GFAP were not found in the ciliary epithelial cells, whereas actin filaments occurred in both cell layers in all regions.
Similar content being viewed by others
References
Coca-Prados M, Chatt G (1986) Growth of non-pigmented ciliary epithelial cells in serum-free hormone-supplemented media. Exp Eye Res 43:617–629
Coca-Prados M, Kondo K (1985) Separation of bovine pigmented ciliary epithelial cells by density gradient and further characterization in culture. Exp Eye Res 40:731–739
Coca-Prados M, Lôpez-Briones LG (1987) Evidence that the α and α(+) isoforms of the catalytic subunit of Na+/K+-ATPase reside in distinct ciliary epithelial cells of the mammalian eye. Biochem Biophys Res Commun 145:460–466
Dahl D, Bignami A (1982) Immunohistological localization of desmin, the muscle-type 100 Å filament protein, in rat astrocytes and Müller glia. J Histochem Cytochem 3:207–213
Duhamel RC, Johnson DA (1985) Use of nonfat dry milk to block nonspecific nuclear and membrane staining by avidin conjugates. J Histochem Cytochem 33:711–714
Eichhorn M, Flügel C, Lütjen-Drecoll E (1990) Regional differences in the bovine ciliary body. An ultrastructural and histochemical study. Fortschr Ophthalmol 87:241–246
Eichhorn M, Inada K, Lütjen-Drecoll E (1991) Human ciliary body in organ culture. Curr Eye Res 4:277–286
Hara K, Lütjen-Drecoll E, Prestele H, Rohen JW (1977) Structural differences between regions of the ciliary body in primates. Invest Ophtahlmol Vis Sci 16:912–924
Helbig H, Korbmacher C, Wiederholt M (1987) K+-Conductance and electrogenic Na+/K+-transport of cultured bovine pigmented ciliary epithelium. J Membrane Biol 99:173–186
Helbig H, Korbmacher C, Erb C, Nawrath M, Knuutila KG, Wistrand P, Wiederholt M (1989) Carbonic anhydrase and its role in ion transport in cultured pigmented ciliary epithelial cells. Fortschr Ophthalmol 86:474–477
Johnson GD, Davidson RS, Me Namee KC, Russell G, Goodwin D, Holborow EJ (1982) Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy. J Immunol Methods 55:231–242
Kasper M (1991) Heterogeneity in the immunolocalization of cytokeratin specific monoclonal antibodies in the rat eye: evaluation of unusual epithelial tissue entities. Histochemistry 95:613–620
Kasper M, Goertchen R, Stosiek P, Perry G, Karsten U (1986) Coexistence of cytokeratin, vimentin and neurofilament protein in human choroid plexus. An immunohistochemical study of intermediate filaments in neuroepithelial tissues. Virchows Arch [A] 410:173–177
Kasper M, Münchow T, Stosiek P, Karsten U (1987) Similarities in the composition of intermediate filaments between human ciliary and choroid plexus epithelia — correlation with similar secretory functions. Acta Histochem 82:136–137
Kasper M, Moll R, Stosiek P, Karsten U (1988) Patterns of cytokeratin and vimentin expression in the human eye. Histochemistry 89:369–377
Kivelä T, Tarkkanen V, Virtanen I (1986) Intermediate filaments in the human retina and retinoblastoma: an immunohistochemical study of vimentin, glial fibrillary acidic protein, and neurofilaments. Invest Ophthalmol Vis Sci 27:1075–1084
Kondo K, Coca-Prados M, Sears M (1983) Ciliary epithelia of the mammalian eye in cultured explants. Cell Tissue Res 233:629–638
Kondo K, Coca-Prados M, Sears M (1984) Human ciliary epithelia in monolayer culture. Exp Eye Res 38:423–433
Lütjen-Drecoll E (1982) Functional morphology of the ciliary epithelium. In: Lütjen-Drecoll E (ed) Basic aspects of glaucoma Research. Schattauer, Stuttgart, pp 69–87
Lütjen-Drecoll E, Lönnerholm G, Eichhorn M (1983) Carbonic anhydrase distribution in the human and monkey eye by light and electron microscopy. Graefe's Arch Clin Exp Ophthalmol 220:285–291
May CN, Stanley RS, Hitoshi S (1988) Bovine non-pigmented and pigmented ciliary epithelial cells in culture: comparison of catalase, superoxidase dismutase and glutathione peroxidase activities. Exp Eye Res 46:919–928
McKechnie NM, Boulton M, Robey HL, Savage FJ, Grierson I (1988) The cytoskeletal elements of human retinal pigment epithelium: in vitro and in vivo. J Cell Sci 91:303–312
Me Lean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative: a new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Molnar ML, Stefansson K, Marton LS, Tripathi RC, Molnar GK (1984) Distribution of S-100 protein and glial fibrillary acidic protein in normal and gliotic human retina. Exp Eye Res 38:27–34
Ober M, Rohen JW (1979) Regional differences in the line structure of the ciliary epithelium related to accommodation. Invest Ophthalmol Vis Sci 18:655–664
Owaribe K, Kartenbeck J, Rungger-Brdndle E, Franke WW (1988) Cytoskeletons of retinal pigment epithelial cells: interspecies differences of expression patterns indicate independence of cell function from the specific complement of cytoskeletal proteins. Cell Tissue Res 254:301–315
Runyan TE, McCombs WB, Holton OD, McCoy CE, Morgan MP (1983) Human ciliary body epithelium in culture. Invest Ophthalmol Vis Sci 24:687–696
Author information
Authors and Affiliations
Additional information
This investigation was supported by grant Dre 124/3-1 from the Deutsche Forschungsgemeinschaft (DFG)
Correspondence to: M. Eichhorn
Rights and permissions
About this article
Cite this article
Eichhorn, M., Flügel, C. & Liitjen-Drecoll, E. Regional differences in the distribution of cytoskeletal filaments in the human and bovine ciliary epithelium. Graefe's Arch Clin Exp Ophthalmol 230, 385–390 (1992). https://doi.org/10.1007/BF00165951
Issue Date:
DOI: https://doi.org/10.1007/BF00165951