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NMR spectroscopy of recombinant chinese hamster ovary (CHO) cells in a packed bed reactor

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Biotechnology Techniques

Summary

Several clones of CHO cells, including recombinant cell lines expressing Hepatitis B surface antigen, were grown in macroporous collagen microspheres. These provided sufficient cell density in a packed bed recirculation system for phosphorus-31 nuclear magnetic resonance spectroscopic estimation of metabolite concentration. Intracellular nucleoside triphosphate as well as nucleoside tri- plus diphosphate levels were higher in the methotrexate-selected clones compared to the dhfr− cell line.

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Authors and Affiliations

  1. Department of Chemical Engineering, California Institute of Technology, 91125, Pasadena, California, USA

    Girish J. Pendse, Gerard Riedy & James E. Bailey

Authors
  1. Girish J. Pendse
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  2. Gerard Riedy
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  3. James E. Bailey
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Pendse, G.J., Riedy, G. & Bailey, J.E. NMR spectroscopy of recombinant chinese hamster ovary (CHO) cells in a packed bed reactor. Biotechnol Tech 5, 193–198 (1991). https://doi.org/10.1007/BF00152780

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  • DOI: https://doi.org/10.1007/BF00152780

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