Abstract
By fusing a human hybridoma producing an IgG2ķ antibody against human A431 epidermoid carcinoma cells with an Epstein-Barr virus-transformed human B lymphocyte producing an IgG2ķ antibody against Pseudomonas aeruginosa exotoxin A, we established a hybrid hybridoma producing a bispecific monoclonal antibody reacting with both A431 cells and the exotoxin. Human IgG was purified from the culture supernatant of the hybrid hybridoma, and the bispecific monoclonal antibody in the IgG preparation was further separated from the two parental antibodies by hydroxyapatite high-performance liquid chromatography. The human bispecific monoclonal antibody thus obtained efficiently targeted the antibody-reative cells, A431, for attack by the exotoxin in vitro.
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Abbreviations
- bs mAb:
-
Bispecific Monoclonal Antibody
- HRP:
-
Horseradish Peroxidase
- MHA:
-
Mixed Hemadsorption Assay
- MTT:
-
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide
- PEA:
-
Pseudomonas aeruginosa Exotoxin A
- PEG:
-
Polyethylene Glycol
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Honda, S., Ichimori, Y. & Iwasa, S. A hyman hybrid hybridoma producing a bispecific monoclonal antibody that can target tumor cells for attack by Pseudomonas aeruginosa exotoxin A. Cytotechnology 4, 59–68 (1990). https://doi.org/10.1007/BF00148811
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DOI: https://doi.org/10.1007/BF00148811