Abstract
A cluster of genes corresponding to the early ecdysone stimulated puff 2B of the Drosophila melanogaster X chromosome has been localized using in situ hybridization in eight Drosophila species. Genes ecs, dor and swi from this cluster have been mapped in D. funebris, D. virilis, D. hydei, D. repleta, D. mercatorum and D. paranaensis to the telomeric region of the X chromosome, in D. kanekoi to the distal region, and in D. pseudoobscura, to the proximal region of the X chromosome. It is assumed that organization of this cluster in these species is conserved. In D. hydei, multiple hybridization sites of certain DNA probes from this region were found.
Similar content being viewed by others
References
Ananiev, E. V., V. E.Barsky, Yu. V.Ilyin & M. V.Ryzic, 1984. Multiple structural genes of Drosophila melanogaster with varying location in chromosomes. IX. Mapping of location sites of 12 mobile genetic elements in polytene chromosomes. Genetika (USSR) 20: 1942–1952.
Belyaeva, E. S., M. G.Aizenzon, I.Kiss, T. D.Gorelova, S. D.Pak, G. H.Umbetova, P. G. N.Kramers & I. F.Zhimulev, 1982. Mutations and chromosome rearrangements in the 1E3–4–2B18 region of Drosophila melanogaster X chromosome. Dros. Inform. Serv. 58: 184–190.
Belyaeva, E. S., M. G.Aizenzon, V. F.Semeshin, I.Kiss, K.Koczka, E. M.Baricheva, T. D.Gorelova & I. F.Zhimulev, 1980. Cytogenetical analysis of the 2B3–4- 2B11 region of the X-chromosome of Drosophila melanogaster. 1. Cytology of the region and mutant complementation groups. Chromosoma 81: 281–306.
Belyaeva, E. S., M. O.Protopopov, E. M.Baricheva, V. F.Semeshin, M. L.Izquierdo & I. F.Zhimulev, 1987. Cytogenetic analysis of region 2B3–4- 2B11 of the X-chromosome of the Drosophila melanogaster. VI. Molecular and cytological mapping of the ecs locus and 2B puff. Chromosoma 95: 295–310.
Belyaeva, E. S., I. E.Vlassova, Z. M.Biyasheva, V. T.Kakpakov, G.Richards & I. F.Zhimulev, 1981. Cytogenetic analysis of the 2B3–4- 2B11 region of the X chromosome of Drosophila melanogaster. II. Changes in 20-OH ecdysone puffing caused by genetic defects of puff 2B5. Chromosoma 84: 207–219.
Belyaeva, E. S. & I. F.Zhimulev, 1982. Cytogenetic analysis of the X chromosome region 2B3–4- 2B11 of Drosophila melanogaster. IV. Mutation at the swi (singed wings) locus interfering with the late 20-OH ecdysone puff system. Chromosoma 86: 251–263.
Biyasheva, Z. M., E. S.Belyaeva & I. F.Zhimulev, 1985. Cytogenetic analysis of the X-chromosome region 2B1–2–2B9–10 of Drosophila melanogaster. V. Puffing disturbances carried by lethal mutants of gene dor due to ecdysterone deficiency. Chromosoma 92: 351–356.
Chao, A. T. & G. M.Guild, 1986. Molecular analysis of the ecdysterone-inducible 2B5 ‘early’ puff in Drosophila melanogaster. The EMBO J. 5: 143–150.
Dowset, A. P. & W.Young, 1982. Differing level of dispersed repetitive DNA among closely related species of Drosophila. Proc. Nat. Acad. Sci. USA 79: 4570–4574.
Dowset, A. P., 1983. Closely related species of Drosophila can contain different libraries of middle repetitive DNA sequences. Chromosoma 88: 104–108.
Gall, J. C. & M.-L.Pardue, 1971. Nucleic acid hybridization in cytological preparations. Meth. Enzymol. 21: 470–480.
Kokoza, E. B., T. Yu.Kozlova, G. H.Umbetova, E. B.Dubrovsky, V.Pirrotta & I. F.Zhimulev, 1990. Molecular-cytogenetic analysis of the Drosophila melanogaster X chromosome 10A1–2 band. Genetika (USSR) 26: 1361–1369.
Mazina, O. M., E. S.Belyaeva & I. F.Zhimulev, 1991. Cytogenetical analysis of the 2B3–4- 2B11 region of the X-chromosome of Drosophila melanogaster. VII. Influence of the ecs locus on female fertility. Mol. Gen. Genet. 225: 99–105.
Pardue, M. L., 1990. In situ hybridization. Acta histochem. cytochem. 23: 1–16.
Protopopov, M. O., E. S.Belyaeva, E. B.Kokoza, E. M.Sharapova, E. M.Baricheva, O. V.Demakova, V. E.Graphodatskaya & I. F.Zhimulev, 1988. Cytogenic analysis of 2B1–2–2B9–10 of the X chromosome of Drosophila melanogaster. VII. Cloning of the ecs, dor and swi loci. Genetika (USSR) 24: 1614–1623.
Protopopov, M. O., E. S.Belyaeva, I. V.Tretyakova & I. F.Zhimulev, 1991. Molecular map of the 2B region of D. melanogaster X chromosome. Dros. Inform. Serv. 70: 182–184.
Throckorton, L. H., 1975. The phylogeny, ecology and geography of Drosophila. In: Handbook of genetics (King, R. C. N. Y., ed.), Plenum Publ. Co. V.5, p. 421–469.
Vashakidze, R. P., N. A.Mamulashvili & I. Yu.Gabriadze, 1990. Structural and functional organisation of poly(dG-dT)/(dC-dA) sequences in the genome of eukaryotic organisms (a review). Molekulyarnaya Biologia (USSR) 23: 1–16.
Zhimulev, I. F., E. S.Belyaeva, O. V.Fomina, M. O.Protopopov & V. N.Bolshakov, 1986. Cytogenetic and molecular aspects of position effect variegation in Drosophila melanogaster. I. Morphology and genetic activity of the 2AB region in chromosome rearrangement T(1;2)dor var7 Chromosoma 94: 492–504.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kokoza, F.B., Belyaeva, E.S. & Zhimulev, I.F. Localization of genes ecs, dor and swi in eight Drosophila species. Genetica 87, 79–85 (1992). https://doi.org/10.1007/BF00120996
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00120996