Abstract
Best callus initiation was obtained when single-node explants of Fuchsia hybrida were incubated in the light on Gamborg B5 medium containing 5×10-6 M indoleacetic acid and benzylaminopurine at 5×10-7 M or 10-6 M. Healthy callus proliferation was maintained in darkness on full-strength B5 medium supplemented with 5×10-6 M IAA and 5×10-7 M BAP. Regeneration from callus was obtained in 3 to 6 weeks, using half-strength hormone-free Campbell & Durzan medium.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2,4 dichlorophenoxyacetic acid
- IAA:
-
indoleacetic acid
- IBA:
-
indolebutyric acid
- NAA:
-
α-naphthaleneacetic acid
- SE:
-
standard error
References
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Chow, Y.N., Harvey, B.M.R. & Selby, C. An improved method for callus proliferation and regeneration of Fuchsia hybrida . Plant Cell Tiss Organ Cult 22, 17–20 (1990). https://doi.org/10.1007/BF00043693
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DOI: https://doi.org/10.1007/BF00043693