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A plant regeneration protocol from callus cultures of medicinal plant Rhodomyrtus tomentosa

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Abstract

Rhodomyrtus tomentosa (Ait.) Hask is a multifunctional shrub of the Myrtaceae, which uniquely combines ornamental, medicinal, and health purposes. In this study, for the first time, a regeneration system was established in R. tomentosa. Callus was induced on Murashige and Skoog (MS) medium with 0.5 mg L−1 6-benzyladenine (BA), 0.05 mg L−1 α-naphthaleneacetic acid (NAA), and 0.005 mg L−1 thidiazuron (TDZ), from tender leaves and stems. Light yellow-green, rigidity, granule callus was induced, 93.33% of which could differentiate into adventitious shoots within 60 d. The optimal shoot proliferation medium was 1/2 MS medium with 6-BA 1 mg L−1 and NAA 0.1 mg L−1, and cultured in high temperature high light intensity (26 °C, 120 µmol m−2s−1) got the highest rate (86.67%) for regeneration response within 60 days. Adventitious roots exhibited the highest rate at 100% on PGR-free 1/2 MS medium. Within 2 months, over 90% of plantlets survived when transferred to a substrate of sand/soil (1:2 ratio).

Key message

This study provides an efficient protocol to regenerate the multifunctional shrub rose myrtle (Rhodomyrtus tomentosa) from embryogenic calli induced from tender leaves and stems.

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Data availability

All data generated or analyzed during this study are included in this published article.

Abbreviations

NAA:

Naphtha acetic acid

6-BA:

6-Benzyladenim

TDZ:

Thidiazuron

PGRs:

Plant growth regulators

IBA:

3-Indolebutyric acid

ANOVA:

Analysis of variance

MS medium:

Murashige and Skoog (1962) medium

GA3 :

Gibberellic acid

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Funding

This study was supported by the Guangzhou Innovation Leading Team Project (No. 202009020004), “One Center and Three Bases” Project for Flora and Fauna Conservation of Guangdong Province and Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0408).

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Authors and Affiliations

  1. CAS Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Ling Yang, Kunlin Wu, Shengxiang Qiu & Shulin Deng

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Ling Yang & Shulin Deng

  3. Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, Key Laboratory of Carbon Sequestration in Terrestrial Ecosystem, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Honglin Cao

  4. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458, China

    Honglin Cao

  5. Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou, 510650, China

    Shulin Deng

Authors
  1. Ling Yang
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  2. Kunlin Wu
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  3. Shengxiang Qiu
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  4. Honglin Cao
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Contributions

Conceptualization of research: SD and SQ; Designing of the experiments: SD, LY and KW; Contribution of experimental materials: HC and SQ; Execution of field/lab experiments and data collection: LY and KW; Analysis of data and interpretation SD, LY, and KW; Preparation of manuscript: LY and SD.

Corresponding author

Correspondence to Shulin Deng.

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Communicated by Klaus Eimert.

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Yang, L., Wu, K., Qiu, S. et al. A plant regeneration protocol from callus cultures of medicinal plant Rhodomyrtus tomentosa. Plant Cell Tiss Organ Cult 153, 307–317 (2023). https://doi.org/10.1007/s11240-023-02464-z

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  • DOI: https://doi.org/10.1007/s11240-023-02464-z

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