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Callus induction and indirect regeneration of Thunbergia coccinea Wall.

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Abstract

In vitro regeneration of Thunbergia coccinea Wall. ex D. Don via organogenesis was obtained from leaf callus tissue of T. coccinea, a perennial climbing medicinal vine plant with attractive red scarlet blooming in particular season. Highest weighted callus was obtained from leaves of T. coccinea on Murashige and Skoog basal medium supplemented with 5.0 mg/L NAA. Embryogenic callus were observed on MS basal media containing NAA rather than 2,4-D. Somatic embryogenesis was demonstrated by field emission scanning electron microscopic studies of some embryogenic callus. Highest frequency of somatic embryos was obtained in 3.0 mg/L NAA. Indirect shoot bud organogenesis and rhizogenesis were also observed on some embryogenic callus. Plantlets were regenerated from both somatic embryos and shoot buds. Highest number of shoots was noted in 0.1 mg/L NAA and 2.0 mg/L BAP combination while transferring in regeneration media. Regenerated plantlets were hardened successfully on pot. The survival rate of regenerated plants after hardening was 50%.

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Abbreviations

MS:

Murashige and Skoog

NAA:

α-Naphthalene acetic acid

2,4-D:

2,4-Dichlorophenoxyacetic acid

BAP:

Benzyl amino purine

FESEM:

Field emission scanning electron microscope

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Acknowledgements

Authors would like to acknowledge the Department of Biotechnology, The University of Burdwan for laboratory and instrumental assistance and also would like to acknowledge DST PURSE, Government of India for special instrumental assistance. They are grateful to the University Grants Commission of India for financial assistance.

Funding

Maulana Azad National Fellowship Award no. MANF-2017-18-WES-82383 provided by University Grant Commission.

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Authors and Affiliations

  1. Department of Biotechnology, The University of Burdwan, Golapbag, West Bengal, 713104, India

    Kaniz Wahida Sultana & Indrani Chandra

  2. Department of Microbiology, M.U.C Women’s College, Burdwan, West Bengal, India

    Anindita Roy

Authors
  1. Kaniz Wahida Sultana
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  2. Indrani Chandra
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  3. Anindita Roy
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Contributions

Dr. Indrani Chandra designed the experiments. Kaniz Wahida Sultana executed the experiments and analyzed the data statistically. They prepared the manuscript and Dr. Anindita Roy contributed to carry out this experimental research substantially.

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Correspondence to Indrani Chandra.

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All authors approved the manuscript after reading it and declared that there is no conflict of interest.

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Sultana, K.W., Chandra, I. & Roy, A. Callus induction and indirect regeneration of Thunbergia coccinea Wall.. Plant Physiol. Rep. 25, 58–64 (2020). https://doi.org/10.1007/s40502-020-00501-z

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