Abstract
Incompatible (self) pollen tube growth in styles of six cultivars of L. longiflorum is strikingly influenced by treatment of the styles for six minutes in 50°C distilled water prior to pollination. In pretreated detached styles the incompatible pollen tubes reach lengths normally attained by compatible pollen whereas in untreated styles they grow to only approximately two-thirds the length normally reached by compatible tubes. Pretreatment at either 25°C or 45°C for six minutes does not affect incompatible pollen tube growth. Both compatible and incompatible tubes grow only a few millimeters into styles treated at 55°C for six minutes. Stigmatoid cells in these styles appear necrotic. Pretreatment of attached styles at 50°C for six minutes resulted in fruit set following self-pollination. Seed number per capsule ranged from 6 to 114. Stylar pretreatment appears to be a highly promising approach to further investigation concerning the nature of the self-incompatibility reaction. It also appears to offer a suitable means of obtaining seed from self-incompatible plants.
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Paper No. 1096 from the Laboratory of Genetics, University of Wisconsin. Supported in part by a grant from the National Science Foundation.
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Hopper, J.E., Ascher, P.D. & Peloquin, S.J. Inactivation of self-incompatibility following temperature pretreatments of styles in Lilium longiflorum . Euphytica 16, 215–220 (1967). https://doi.org/10.1007/BF00043457
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DOI: https://doi.org/10.1007/BF00043457