Summary
Randomly amplified polymorphic DNA (RAPD) markers combined with the Simple Matching index for calculating the distance matrix were used to determine the genetic diversity of African cassava clones. A preliminary study of three Manihot species using 20 primers clearly showed that RAPD markers were relevant for analyzing their genetic diversity. DNAs from 19 cultivars of Manihot esculenta Crantz (cassava) were amplified using 8 primers. Cultivars were well discriminated and coherently distributed, comparing to previous results from isozymes and RFLP studies. Genetic diversity analysis, characterization of collections and study of introgression are the domains that RAPD markers can contribute to the improvement of cassava.
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Abbreviations
- PCR:
-
Polymerase Chain Reaction
- RAPD:
-
Randomly Amplified Polymorphic DNA
- RFLP:
-
Restriction Fragment Length Polymorphism
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Marmey, P., Beeching, J.R., Hamon, S. et al. Evaluation of cassava (Manihot esculenta Crantz) germplasm collections using RAPD markers. Euphytica 74, 203–209 (1993). https://doi.org/10.1007/BF00040402
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DOI: https://doi.org/10.1007/BF00040402