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Heat shock response during anther culture of broccoli (Brassica oleracea var italica)

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Abstract

Embryo formation from microspores of Brassica oleracea var Italica (Broccoli) and other Brassica species is greatly enhanced by an initial incubation at elevated temperatures (eg 35°C) followed by continued incubation of 25°C. In the present study we observed that a three hour high temperature treatment induced the formation of heat shock proteins in cultured anthers. These were identified in two dimensional gels by silver staining, and labelled heat shock proteins were synthesised in vitro from isolated anther RNA. The appearance of heat shock proteins in anthers followed a similar pattern and displayed similar characteristics to that from leaves. Comparison of the heat shock proteins induced in isolated cultured anthers of known highly embryogenic and less embryogenic plans did not reveal obvious qualitative differences.

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Authors and Affiliations

  1. Paladin Hybrids Inc., 2475 Don Reid Drive, K1H 8P5, Ottawa, Ontario, Canada

    Steven F. Fabijanski & Paul G. Arnison

  2. The Biochemistry Dept., The University of Ottawa, 40 Marie Curie Drive, K1N 9B4, Ottawa, Ontario, Canada

    Illimar Altosaar

Authors
  1. Steven F. Fabijanski
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  2. Illimar Altosaar
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  3. Paul G. Arnison
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Fabijanski, S.F., Altosaar, I. & Arnison, P.G. Heat shock response during anther culture of broccoli (Brassica oleracea var italica). Plant Cell Tiss Organ Cult 26, 203–212 (1991). https://doi.org/10.1007/BF00039945

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  • DOI: https://doi.org/10.1007/BF00039945

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