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Elicitor-mediated induction of enzymes of lignin biosynthesis and formation of lignin-like material in a cell suspension culture of spruce (Picea abies)

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Abstract

Incubations of photomixotrophic suspension culture cells of spruce (Picea abies) (L.) (Karst) with an autoclaved cell wall preparation of Rhizosphaera kalkhoffii as elicitor led to a rapid increase of the activity of a number of enzymes involved in lignin biosynthesis. l-phenylalanine ammonia-lyase (EC 4.3.1.5) was induced about 10-fold, feruloyl-Coenzyme A reductase (ED 1.2.1.44) 4-fold, cinnamyl alcohol dehydrogenase (NADP+) (EC 1.1.1.195) 2-fold and peroxidase (EC 1.11.1.7) about 1.5-fold. The induction of the enzymes, with the exception of the peroxidase, was transient, showing maximal activity within 3 days after elicitation. Extracellular peroxidase activity, determined in the culture medium, rapidly decreased on initiation of elicitation.

Concomitant with the increase of activity of the enzymes of lignin synthesis was a rapid clouding of the culture medium. Phloroglucinol-HCl staining revealed the presence of lignin-like material in the medium and also in the cells. The IR-spectrum of this material was identical with the IR-spectrum of authentic spruce lignin.

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Abbreviations

PAL:

l-phenylalanine ammonia-lyase

FCR:

feruloyl-Coenzyme A reductase

CAD:

cinnamyl alcohol dehydrogenase

POD:

peroxidase

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  1. Abt.Zellchemie, G.S.F., National Center for Environment and Health, München, P.O. Box 1129, D-85758, München-Oberschleissheim, Germany

    Burkhard Messner & Meinrad Boll

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  1. Burkhard Messner
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  2. Meinrad Boll
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Messner, B., Boll, M. Elicitor-mediated induction of enzymes of lignin biosynthesis and formation of lignin-like material in a cell suspension culture of spruce (Picea abies). Plant Cell Tiss Organ Cult 34, 261–269 (1993). https://doi.org/10.1007/BF00029715

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  • DOI: https://doi.org/10.1007/BF00029715

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