Abstract
Levels of endogenous growth substances (abscisic acid: ABA; indole-3-acetic acid: IAA) and applied benzyladenine (BA) were quantified during the eight first days of in vitro propagation of Wild Cherry (Prunus avium L.). Axillary buds from the middle part of the explants started to grow at day 2, thus were released from apical dominance. Hormone levels were measured in the apical, median and basal parts of the explants using an avidin-biotin based enzyme-linked immunosorbent assay (ELISA) after a purification of the extracts by high performance liquid chromatography (HPLC). All hormones showed rapid and considerable changes during the first eight days of growth. Exogenous IBA was probably transformed into IAA mainly in the basal part of the explant, and BA penetrated quickly. ABA levels were transiently enhanced in the apical part of the explants bearing young leaves. These phenomena are discussed in connection with the axillary bud reactivation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bengtson C, Klockare B, Klockare R, Larsson S and Sundqvist C 1978 The after-effect of water stress on chlorophyll formation during greening and the levels of abscisic acid and proline in dark grown wheat seedlings. Physiol Plant 43:205–212
Biondi S, Canciani L and Bagni N 1984 Uptake and translocation of benzyladenine by elm shoots cultured in vitro. Can J Bot 62:2385–2390
Cornu D and Boulay M 1986 La multiplication végétative. Techniques horticoles et culture in vitro. RFF 38:60–68
Epstein E and Lavee S 1986 Conversion of indole-3-butyric acid to indole-3-acetic acid by cuttings of grapevine (Vitis vinifera) and olive (Olea europea). Plant and Cell Physiol 25:697–703
Hartung W and Steigerwald F 1977 Abscisic acid and apical dominance in Phaseolus coccineus L. Planta 299:295–299
Hicks GS and Nair A 1986 Growth and morphogenesis in short-term nodal cultures of an apple rootstock in vitro. Can J Bot 64:2299–2304
Hillman JR 1986 Apical dominance and correlations by hormones. In: MBopp, ed. Plant Growth Substances, pp. 341–349. Berlin, Heidelberg: Springer Verlag
Knox JP and Wareing PF 1984 Apical dominance in Phaseolus vulgaris L.: the possible roles of abscisic and indole-3-acetic acid. J Exp Bot 35;239–244
Label P and Sotta B 1988 An ELISA test for BA measurement in Wild Cherry cultured in vitro. Plant Cell, Tissue Organ Culture, 13: 155–158
Letham DS and Palni LMS 1983 The biosynthesis and metabolism of cytokinins. Annu Rev Plant Physiol 34:163–197
Maldiney R, Leroux B, Sabbagh I, Sotta B, Sossountzov L and Miginac E 1986 A biotin-avidin-based enzyme immunoassay to quantify three phytohormones: auxin, abscisic acid and zeatin-riboside. J Immunol Meth 90:151–158
Miginiac E 1974 Quelques aspects morphologiques, physiologiques et biochimiques de la dominance apicale. Physiol Vég 12:689–720
Nordström AC and Eliasson L 1986 Uptake and translocation of [14C]-labelled benzyla-minopurine in apple shoots grown in vitro in relation to shoot development. Physiol Plant 68:431–435
Phillips DJ 1975 Apical dominance. Annu Rev Plant Physiol 26:341–367
Pillay I and Railton ID 1983 Complete release of axillary buds from apical dominance in intact, light-grown seedlings of Pisum sativum L. following a single application of cytokinin. Plant Physiol 71:972–974
Riffaud JL and Cornu D 1981 Utilisation de la culture in vitro pour la multiplication de merisiers adultes (Prunus avium L.) sélectionnés en forêt. Agronomie 1:633–640
Saniewski M and Puchalski J 1987 The effect of methyl jasmonate and abscisic acid on differentiation of benzyladenine-induced bulblets in Muscari bulbs. Biol Plant 29:63–65
Sotta B, Pilate G, Pelese F, Bonnet M and Maldiney R 1987 An avidin-biotin solid phase ELISA for femtomol isopentenyladenine and isopentenyladenosine measurements in HPLC purified plant extracts. Plant Physiol 84:571–573
Vogelmann TC, Bornmann CH and Nissen P 1984 Uptake of benzyladenine in explants of Picea abies and Pinus sylvestris Physiol Plant 61:513–517
Walton DC 1980 Biochemistry and physiology of abscisic acid. Annu Rev Plant Physiol 31:453–489
Welander M 1985 In vitro shoot and root formation in the apple cultivar Akero. Ann Bot 55:249–261
Wooley DJ and Wareing PF 1972 The interaction between growth promoters in apical dominance. II. Environmental effects on endogenous cytokinin and gibberellin levels in Solanum andigena. New Phytol 71:1015–1025
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Label, P., Maldiney, R., Sossountzov, L. et al. Endogenous levels of abscisic acid, indole-3-acetic acid and benzyladenine during in vitro bud growth induction of Wild cherry (Prunus avium L.). Plant Growth Regul 7, 171–180 (1988). https://doi.org/10.1007/BF00028239
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00028239