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Nucleotide sequence and phylogenetic implication of the ATPase subunits β and ε encoded in the chloroplast genome of the brown alga Dictyota dichotoma

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Abstract

We have cloned and sequenced the genes atpB and atpE, coding for CF1 subunits β and ε, respectively, of the chloroplast genome of the brown alga Dictyota dichotoma. Although the coding site of atpE cannot be demonstrated by heterologous Southern hybridizations, a 417 bp reading frame 3′ to atpB was identified as the gene atpE by sequence similarities with atpE genes from other sources. A maximum sequence identity of 30% is found between the predicted amino acid sequence of the Dictyota subunit ε and the corresponding cyanobacterial subunits. Including conserved amino acid replacements, the Dictyota ε subunit exhibits about 70% sequence similarity with the cyanobacterial and land plant subunits. As in cyanobacteria, the atpE gene does not overlap the preceding gene atpB. The deduced amino acid sequence of atpB is 74–79% identical to the corresponding cyanobacterial and chloroplast subunits. Entirely conserved are regions referred to as the catalytic and/or regulatory sites of ATP formation, including interacting regions between subunits α and β. A phylogram predicted from F1/CF1-β subunits of eleven different organisms suggests a common evolutionary origin of plastids from chlorophytes and brown algae.

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  1. Institut für Botanik, Heinrich-Heine Universität Düsseldorf, Universitätstrasse 1, D-4000, Düsseldorf 1, Germany

    Carola E. W. Leitsch & Klaus V. Kowallik

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  1. Carola E. W. Leitsch
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  2. Klaus V. Kowallik
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Leitsch, C.E.W., Kowallik, K.V. Nucleotide sequence and phylogenetic implication of the ATPase subunits β and ε encoded in the chloroplast genome of the brown alga Dictyota dichotoma . Plant Mol Biol 19, 289–298 (1992). https://doi.org/10.1007/BF00027350

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  • DOI: https://doi.org/10.1007/BF00027350

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