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Oncogene arrangement in a shooty strain of Agrobacterium tumefaciens

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Abstract

The Agrobacterium tumefaciens nopaline strain 82.139 induces non-teratogenic shooty tumours on several plant species. We have determined the position of the T-region oncogenes in a 11.4 kb Xba I fragment which shows a general organization similar to its pTiC58 counterpart. Sequence analysis of the 4.7 kb right part of this fragment allowed us to identify the pTi82.139 ipt, 6b and nos coding sequences. pTi82.139 lacks the 6a gene, which lies between the ipt and 6b genes in pTiC58. The intervening region between the 6b and the nos genes contains an additional ORF with homology to ORF 21 (transcript 3′) from the TR-DNA of octopine strain pTi15955.

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Author notes

  1. Ana Cristina Miranda Brasileiro

    Present address: National Center of Genetic Resources and Biotechnology (CENARGEN/EMBRAPA)-SAIN, Parque Rural, 70.770, Brasíllia, Brazil

Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire, INRA, route de Saint-Cyr, F-78026, Versailles Cedex, France

    Christine Drevet, Ana Cristina Miranda Brasileiro & Lise Jouanin

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  1. Christine Drevet
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  2. Ana Cristina Miranda Brasileiro
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  3. Lise Jouanin
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Drevet, C., Brasileiro, A.C.M. & Jouanin, L. Oncogene arrangement in a shooty strain of Agrobacterium tumefaciens . Plant Mol Biol 25, 83–90 (1994). https://doi.org/10.1007/BF00024200

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  • DOI: https://doi.org/10.1007/BF00024200

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