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Nucleotide sequence, evolutionary origin and biological role of a rearranged cytokinin gene isolated from a wide host range biotype III Agrobacterium strain

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Summary

A DNA fragment with homology to the cytokinin (ipt) gene from biotype I Agrobacterium tumefaciens strain Ach5 was cloned from the Ti plasmid of the wide host range biotype III Agrobacterium strain Tm-4 and sequenced. The fragment contains an intact ipt coding sequence. However, the 3′ non-coding region of this ipt gene is rearranged due to a 0.9 kb deletion fusing it to the 3′ coding region of the neighbouring gene 6a, most of which was found to be deleted. The Tm-4 ipt gene is strongly related to the partially deleted ipt gene of the limited host range biotype III strain Ag162. To test its biological activity, the Tm-4 ipt gene was inserted into a specially constructed, disarmed Ti vector lacking tzs and tested on tobacco, where the rearranged ipt gene induced shoot formation. The cloned Tm-4 ipt gene was mutated with Tn5 and the intact gene on the wild-type Tm-4 Ti plasmid was replaced by the mutated gene. The resulting strain was avirulent on tobacco but normally virulent on the natural host of the wild-type strain Tm-4, grapevine. As the biotype 1 6b gene diminishes the effect of a corresponding ipt gene, a larger Tm-4 fragment carrying both the ipt gene and an adjacent 6b-like gene was also tested on tobacco and compared with the Tm-4 ipt fragment alone and with an ipt and 6b/ipt fragment derived from Ach5. The Tm-4 6b gene diminishes the effect of the Tm-4 ipt gene, showing the Tm-4 6b gene to be active as well. The Tm-4 6b/ipt combination is less effective than the Ach5 combination. These results provide further insight into the molecular basis of the host range differences between limited host range and wide host range biotype III Agrobacterium strains and show that the WHR cytokinin gene, although active, does not significantly contribute to tumour formation on the natural host of the WHR biotype III strains, grapevine.

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Abbreviations

LHR:

limited host range

WHR:

wide host range

onc :

oncogenicity genes

iaaH :

indoleacetamide hydrolase gene

iaaM :

tryptophan monooxygenase gene

ipt :

isopentenyl transferase gene

tzs :

transzeatin secretion gene

NAA:

α-naphthalene acetic acid

BAP:

6-benzylaminopurine

Km:

kanamycin

Neo:

neomycin

Cm:

chloramphenicol

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Authors and Affiliations

  1. Institut de Biologie Moléculaire des Plantes du CNRS, Rue du Général Zimmer 12, F-67000, Strasbourg, France

    Géraldine Bonnard, Bruno Tinland, François Paulus & Léon Otten

  2. Research Institute of Viticulture and Oenology, P.O. Box 25, 6000, Kecskemét, Hungary

    Ernö Szegedi

Authors
  1. Géraldine Bonnard
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  2. Bruno Tinland
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  3. François Paulus
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  4. Ernö Szegedi
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  5. Léon Otten
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Communicated by J. Schell

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Bonnard, G., Tinland, B., Paulus, F. et al. Nucleotide sequence, evolutionary origin and biological role of a rearranged cytokinin gene isolated from a wide host range biotype III Agrobacterium strain. Mol Gen Genet 216, 428–438 (1989). https://doi.org/10.1007/BF00334387

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