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Improved plant heterokaryon formation by surfactant-supplementation of polyethylene glycol fusogen solution

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Summary

PEG fusion solution for leaf protoplasts of Petunia parodii and cell suspension protoplasts of albino P. hybrida cv. Comanche was supplemented with 0.01–1.0% (w/v) Pluronic F-68. This stimulated protoplast fusion overall, including parental homokaryon formation, with increased means of 23% and 83% respectively, over appropriate controls using 1.0% (w/v) surfactant added to the standard PEG solution. Interestingly, the percentage heterokaryon formation increased near 2-fold (P<0.001) for fusogen solutions supplemented with 0.01% (w/v) Pluronic. Protoplasts regenerated to colonies in KM8P/KM8 liquid medium, indicating no adverse effects of Pluronic F-68 on viability, both in the short and longer terms.

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Abbreviations

BA:

6-benzyladenine

MS:

Murashige & Skoog (1962)

NAA:

α-naphthaleneacetic acid

PEG:

polyethylene glycol

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Author notes

  1. T. K. Hill

    Present address: Department of Life Science, Nottingham Trent University, Clifton Lane, NG11 8NS, Nottingham, U.K.

Authors and Affiliations

  1. Department of Life Science, University of Nottingham, University Park, NG7 2RD, Nottingham, U.K.

    T. K. Hill, M. R. Davey, J. B. Power, P. Anthony & K. C. Lowe

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  1. T. K. Hill
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  2. M. R. Davey
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  3. J. B. Power
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  4. P. Anthony
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  5. K. C. Lowe
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Hill, T.K., Davey, M.R., Power, J.B. et al. Improved plant heterokaryon formation by surfactant-supplementation of polyethylene glycol fusogen solution. Euphytica 85, 269–273 (1995). https://doi.org/10.1007/BF00023955

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