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SDS-PAGE of the high-molecular-weight subunits of wheat glutenin and the characterization of 1R (1B) substitution and 1BL/1RS translocation lines

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Summary

The high-molecular-weight subunits of glutenin from wheat 1R(1B) substitution and 1BL/1RS translocation lines were fractionated by SDS-PAGE. Two new subunits denoted R1 and R2 were characterized in 1R(1B) substitution, but not in 1BL/1RS translocation lines. R1 and R2 were proved to be rye proteins by 2d electrophoresis (NEPHGE x SDS-PAGE).

In contrast to literature citations it was demonstrated that the cultivar Winnetou is a 1R(1B) substitution line and the cultivars Clement and Mildress both are 1BL/1RS translocation lines.

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Authors and Affiliations

  1. Institute for Cereals, Flour and Bread TNO, P.O. Box 15, 6700 AA, Wageningen, the Netherlands

    Johannes H. E. Moonen

  2. Institute of Plant Breeding (IvP), Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, the Netherlands

    Anton C. Zeven

Authors
  1. Johannes H. E. Moonen
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  2. Anton C. Zeven
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Moonen, J.H.E., Zeven, A.C. SDS-PAGE of the high-molecular-weight subunits of wheat glutenin and the characterization of 1R (1B) substitution and 1BL/1RS translocation lines. Euphytica 33, 3–8 (1984). https://doi.org/10.1007/BF00022742

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  • DOI: https://doi.org/10.1007/BF00022742

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