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Molecular profiling of a y-type high molecular weight glutenin subunit at Glu-D1 locus from a North Korean landrace wheat (Triticum aestivum L.)

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Abstract

The objective of this study is to demonstrate characteristics of a y-type high molecular weight glutenin subunit (D1y HMW-GS) at Glu-D1 found in IT212991, a North Korean landrace wheat compared to Dy12 and Dy12.K as a novel HMW-GS in JB20, a Korean wheat line onto molecular analyses as PCR, cloning, DNA sequencing, and RP-HPLC and proteomic analyses as sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE), two-dimensional electrophoresis (2-DE), Fourier-transform mass spectrometry (LTQ-FT-MS). The D1y of IT212991 was identified to have faster electrophoretic mobility than that of Dy12 by SDS–PAGE. HMW-GS components of IT212991 were identified to be different from Chinese Spring (CS) and JB20, a Korean wheat line by RP-HPLC. The result of mass spectrometric analysis, the D1y of IT212991 (68510.8 Da) was similar to that of Dy12.K of JB20 (68514.4 Da), and lower than Dy12 of CS (69151.2 Da). The result of LTQ-FT-MS based on 2-DE, the D1y of IT212991 was identified to be similar with Dy12 corresponding to the protein function as ‘Glutenin, high molecular weight subunit 12’. The D1y encoding the D1y of IT212991 was identified to consist of 652 amino acid sequences corresponding to 1962 bp according to DNA sequencing. The gene was identified to have a insertion and deletion (InDel) corresponding to 18 bp sequences ‘AACAGGACAAGGGCAACA’ compared to ordinary Dy12 gene. It was demonstrated that the D1y of IT212991 is the same as Dy12.K.

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Acknowledgements

This work was carried out with the support of the “Cooperative Research Program for Agriculture Science and Technology Development (RDA PJ01237601)” Rural Development Administration, Republic of Korea.

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  1. Seong-Woo Cho and Kun Cho contributed equally to this work.

Authors and Affiliations

  1. Department of Crop Science and Biotechnology, Chonbuk National University, Jeonju, 54896, Republic of Korea

    Seong-Woo Cho & Chul Soo Park

  2. Biomedical Omics Group, Korea Basic Science Institute, Cheongju, 28119, Republic of Korea

    Kun Cho & Geul Bang

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  1. Seong-Woo Cho
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  2. Kun Cho
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  3. Geul Bang
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  4. Chul Soo Park
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Correspondence to Chul Soo Park.

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Cho, SW., Cho, K., Bang, G. et al. Molecular profiling of a y-type high molecular weight glutenin subunit at Glu-D1 locus from a North Korean landrace wheat (Triticum aestivum L.). Plant Biotechnol Rep 12, 139–148 (2018). https://doi.org/10.1007/s11816-018-0479-6

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