Summary
The location and nucleotide sequence of tobacco chloroplast genes for tRNASer (GGA), tRNAThr (UGU), tRNALeu (UAA) and tRNAPhe (GAA) (trnS-GGA, trnT-UGU, trnL-UAA and trnF-GAA, respectively) have been determined. These genes are located in the 10 kbp BamHI fragment which lies in the middle of the large single-copy region of the chloroplast DNA. The gene order is trnS-trnT-trnL-trnF. The trnS, trnL and trnF are encoded on the same strand while the trnT on the opposite strand. The trnL contains a 503 bp intron like maize and broad bean trnL-UAAs.
Similar content being viewed by others
References
Bergmann P, Seyer P, Burkard G, Weil JH: Mapping of transfer RNA genes on tobacco chloroplast DNA. Plant Mol Biol 3:29–36, 1984.
Bonnard G, Michel F, Weil JH, Steinmetz A: Nucleotide sequence of the split tRNALeu (UAA) gene from Vicia faba chloroplasts: evidence for structural homologies of the chloroplast tRNALeu intron with the intron from the autosplicable Tetrahymena ribosomal RNA precursor. Mol Gen Genet 194:330–336, 1984.
Bonnard G, Weil JH, Steinmetz A: The intergenic region between the Vicia faba chloroplast tRNALeu (CAA) and tRNALeu (UAA) genes contains a partial copy of the split tRNALeu (UAA) gene. Curr Genet. 9:417–422, 1985.
Canaday J, Guillemaut P, Gloeckler R, Weil JH: Comparison of the nucleotide sequences of chloroplast tRNAsPhe and tRNAsLeu from spinach and bean. Plant Sci Lett 20:57–62, 1980.
Chang SH, Brum CK, Silberklang M, RajBhandary UL, Hecker LI, Barnett WE: The first nucleotide sequence of an organelle transfer RNA: chloroplast tRNAPhe. Cell 9:717–723, 1976.
Crouse EJ, Bohnert HJ, Schmitt JM: Chloroplast RNA synthesis. In: Ellis RJ (ed) Chloroplast Biogenesis. Cambridge University Press, Cambridge, 1984, pp 83–136.
Deno H, Sugiura M: The nucleotide sequences of tRNASer (GCU) and tRNAGln (UUG) genes from tobacco chloroplasts. Nucl Acids Res 11:8407–8414, 1983.
Gruissem W, Zurawski G: Identification and mutational analysis of the promoter for a spinach chloroplast transfer RNA gene. EMBO J 4:1637–1644, 1985.
Guillemaut P, Keith G: Primary structure of bean chloroplastic tRNAPhe: Comparison with euglena chloroplastic tRNAPhe. FEBS Lett 84:351–356, 1977.
Hallick RB, Hollingsworth MJ, Nickoloff JA: Transfer RNA genes of Euglena gracilis chloroplast DNA. Plant Mol Biol 3:169–175, 1984.
Kato A, Takaiwa F, Shinozaki K, Sugiura M: Location and nucleotide sequence of the genes for tobacco chloroplast tRNAArg (ACG) and tRNALeu (UAG). Curr Genet 9:405–409, 1985.
Michel F, Dujon B: Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members. EMBO J 2: 33–38, 1983.
Ohme M, Kamogashira T, Shinozaki K, Sugiura M: Locations and sequences of tobacco chloroplast genes for tRNAPro (UGG), tRNATrp, tRNAfMet and tRNAGly (GCC): the tRNAGly contains only two base-pairs in the D stem. Nucl Acids Res 12:6741–6749, 1984.
Osorio-Almeida ML, Guillemaut P, Keith G, Canaday J, Weil JH: Primary structures of three leucine transfer RNAs from bean chloroplast. Biochem Biophys Res Commun 92:102–108, 1980.
Pillay DTN, Guillemaut P, Weil JH: Nucleotide sequences of three soybean chloroplast tRNAsLeu and re-examination of bean chloroplast tRNALeu sequence. Nucl Acids Res 12:2997–3001, 1984.
Steinmetz A, Gubbins EJ, Bogorad L: The anticodon of the maize chloroplast gene for tRNALeu (UAA) is split by a large intron. Nucl acids Res 10:3027–3037, 1982.
Steinmetz AA, Krebbers ET, Schwartz Z, Gubbins EJ, Bogorad L: Nucleotide sequences of five maize chloroplast transfer RNA genes and their flanking regions. J Biol Chem 258:5503–5511, 1983.
Shinozaki K, Deno H, Sugita M, Kuramitsu S, Sugiura M: Intron in the gene for the ribosomal protein S16 of tobacco chloroplast and its conserved boundary sequences. Mol Gen Genet in press, 1985.
Sugita M, Shinozaki K, Sugiura M: Tobacco chloroplast tRNALys (UUU) gene contains a 2.5-kilobase-pair intron: An open reading frame and a conserved boundary sequence in the intron. Proc Natl Acad Sci USA 82:3557–3561, 1985.
Sugiura M, Kusuda J: Molecular cloning of tobacco chloroplast ribosomal RNA genes. Mol Gen Genet 172:137–141, 1979.
Sugiura M, Shinozaki K, Ohme M: Tobacco chloroplast genes for transfer RNAs. In: Vloten-Doting L, Groot GSP, Hall TC (eds) Molecular Form and Function of the Plant Genome. Plenum Publishing Corp, New York, 1985, pp 325–334.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamada, K., Shinozaki, K. & Sugiura, M. DNA sequences of tobacco chloroplast genes for tRNASer (GGA), tRNAThr (UGU), tRNALeu (UAA), tRNAPhe (GAA): the tRNALeu gene contains a 503 bp intron. Plant Mol Biol 6, 193–199 (1986). https://doi.org/10.1007/BF00021488
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00021488