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Tissue culture of Ecklonia radiata (Phaeophyceae, Laminariales): effects on growth of light, organic carbon source and vitamins

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Abstract

The effects of light quality and irradiance, and supply of organic carbon and vitamins on the growth of two forms of Ecklonia radiata in tissue culture were examined. A callus of unpigmented cells developed over the cut surface of newly excised explants of stipe. This growth was best in the dark but stopped after 10 weeks. Pigmented, mainly filamentous clumps of cells developed from explants after several weeks in culture. These required light for growth, with growth being enhanced by increasing photon flux density up to 30 μmol photon m-2 s-1, with the active spectral component being red light (> 600 nm). The addition to the medium of a range of organic carbon sources or vitamins did not stimulate growth of either culture type in the dark.

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  1. School of Biological and Environmental Sciences, Murdoch University, Murdoch, 6150, Western Australia

    Heather J. Lawlor, Jen A. McComb & Michael A. Borowitzka

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  1. Heather J. Lawlor
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  2. Jen A. McComb
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  3. Michael A. Borowitzka
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Lawlor, H.J., McComb, J.A. & Borowitzka, M.A. Tissue culture of Ecklonia radiata (Phaeophyceae, Laminariales): effects on growth of light, organic carbon source and vitamins. J Appl Phycol 1, 105–112 (1989). https://doi.org/10.1007/BF00003872

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