Abstract
Callus formation of seven species of the agarophyte marine algae Phyllophora nervosa, Gracilaria verrucosa, Furcellaria fastigiata, ceramium kondoi, Gelidium vagum, Laurencia paniculata, Rhodymenia pertusa was obtained from 1984 to 1986. A new sterile agent, chlorhexidin bigluconate, was used to get axenic explants from the algae thalli. The formation of callus from axenic explants depended on temperature of cultivation. In the case of F. fastigiata and G. vagum it occurred at 12° to 20°C, and with P. nervosa at 18° to 26°C. The exchange of sucrose with mannitol in the culture medium and the addition of some growth regulators stimulated the formation of callus. Mass formation of lumpy and warty extensions was observed in the explants from leaf blades of P. nervosa. The results might be useful for improving the aquaculture of agarophyte marine algae.
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Literature cited
Chen, C.-M.: Callus-like formation from Chondrus crispus. First Int. Phycological Cong. St. John's, Newfoundland, Canada, Progr. and Scient. Abstracts, a.8 (1982)
Chen, L. C.-M. and A. R. A. Taylor: Medullary tissue culture of red alga Chondrus crispus. Can. J. Bot. 56, 883–886 (1978)
Fang, T. C.: Some genetic features revealed from culturing the haploid cells of kelps. Hydrobiologia 116/117, 317–318 (1984)
Fries, L.: Growth regulating effects of phenylacetic acid and phydroxyphenylacetic acid on Fucus spiralis L. (Phaeophyceae, Fucales) in axenic culture. Phycologia 16, 451–455 (1977)
Fries, L.: Axenic tissue cultures from the sporophytes of Laminaria digitata and Laminaria hyperborea (Phaeophyta). J. Phycol. 16, 475–477 (1980)
Gusev, M. V., A. H. Tambiev, N. N. Kirikova, R. R. Aslanyan and N. N. Shelyastina. Obtaining of axenic explants from marine algae thallus. (in Russian). Izv. Acad. Nauk USSR ser. Biol. 5, 716–721 (1984)
Murashige, T. and F. Scoog: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497 (1962)
Nakamura, T.: Production of agar. Japan Patent (Kokai) 74-101561 (1974)
Polne-Fuller, M., M. Biniaminov and A. Gilbor: Vegetative propagation of Porphyra perforata. Hydrobiologia, 116/117, 308–313 (1984)
Saga, N., F. Motomura and A. J. Sakai: Induction of callus from marine brown alga Dictyosiphon foeniculaceus. Plant Cell Physiol. 23, 721–730 (1982)
Tambiev, A. H. and R. R. Aslanyan: Explantation of macrophytic algae into artificial nitrient medium. Oceanology, USSR 29, 139–142 (1984)
Wang, S. and Z. Xu.: Observation in the ultrastructure and regeneration in some species of Gracilaria (Rhodophyta). XIth Int. Seaweed Symp., Qingdao, China, Abstracts, p 251. 1983
Yan, Z. M.: Studies on tissue culture of Laminaria japonica and Undaria pinnatifida. Hydrobiologia 116/117, 314–316 (1984)
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Communicated by O. Kinne, Oldendorf/Luhe
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Gusev, M.V., Tambiev, A.H., Kirikova, N.N. et al. Callus formation in seven species of agarophyte marine algae. Mar. Biol. 95, 593–597 (1987). https://doi.org/10.1007/BF00393103
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DOI: https://doi.org/10.1007/BF00393103