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Features of Growth and Inulin Content in Callus Cultures Cichorium intybus L. In Vitro

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Abstract

In vitro callus cultures of common chicory (Cichorium intybus L.) were obtained and their growth and biochemical characteristics depending on the hormonal composition of the MS medium and the spectral composition of light were studied. The study of the effect of light culture on callus tissue formation and inulin accumulation in it was carried out in opaque grow tents with radiation aligned with the flux density of photosynthetic photons and different ratios of its levels in the region of 660 nm (R, red) and 730 nm (FR, far red). The control variant was placed under white linear fluorescent lamps. The resulting cultures were characterized by high proliferative activity and the capability for morphogenesis. It has been established that the interaction of two factors—the presence of auxins in the nutrient medium (IAA or NAA at a concentration of 7.5 mg/L in combination with BAP 0.5 mg/L) and cultivation under light culture conditions (FR > R, FR = R, FR < R)—had a significant impact on the biosynthetic potential of cell cultures. In the obtained cultures, a study of the quantitative content of inulin was carried out. It has been shown that the high content of inulin (7.55–7.95%) in callus cultures was on the MS medium in combination with IAA at FR > R illumination. This is probably due to the fact that well proliferating and highly morphogenic callus tissue was formed under these conditions. The obtained results confirm the hypothesis about the specificity of cultured cells to in vitro synthesize and accumulate secondary metabolites in dedifferentiated cells and the dependence of this process on factors of chemical and physical nature.

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Funding

The work was carried out within the framework of the thematic plan-task for the implementation of research works of the Federal State Budgetary Educational Institution of Higher Education Russian State Agrarian University–Timiryazev Moscow Agricultural Academy commissioned by the Ministry of Agriculture of Russia at the expense of the federal budget in 2023.

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  1. Federal State Budgetary Educational Institution of Higher Education Russian State Agrarian University−Timiryazev Moscow Agricultural Academy, Moscow, Russia

    E. A. Kalashnikova, R. N. Kirakosyan, V. I. Trukhachev, M. G. Pankova & A. V. Sumin

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  1. E. A. Kalashnikova
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  2. R. N. Kirakosyan
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Correspondence to E. A. Kalashnikova.

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Kalashnikova, E.A., Kirakosyan, R.N., Trukhachev, V.I. et al. Features of Growth and Inulin Content in Callus Cultures Cichorium intybus L. In Vitro. Russ J Plant Physiol 70, 78 (2023). https://doi.org/10.1134/S1021443723600320

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