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3 Biotech

, 8:45 | Cite as

Isolation, characterization and exploring biotechnological potential of halophilic archaea from salterns of western India

  • Aparna SinghEmail author
  • Anil Kumar Singh
Original Article

Abstract

Thirteen halophilic archaea were isolated from Kandla and Bhayander salt pans. These isolates were grouped into three different genera Halobacterium, Haloferax and Haloarcula based on morphological and biochemical characterization, polar lipid analysis, Amplified 16S rDNA restriction analysis (ARDRA) and 16S rDNA sequence analysis. Biochemical characterization suggested the ability of isolates to produce protease, amylase and poly-hydroxybutyrate (PHB) indicating their biotechnological potential. The isolates were further screened for the amount of extracellular protease produced. Halobacterium sp. SP1(1) showed significant protease production compared to other isolates. Protease producing ability of the isolate was influenced by several factors such as NaCl concentration, type of protein source, metal ions and surfactants, and presence of amino acid supplements in the production medium. Soybean flour, FeCl3 and dicotylsulfosuccinate were found to increase protease production by 2.36, 1.54 and 1.26 folds, respectively compared to production in basal medium. Effect of organic solvents used in paints (n-decane, n-undecane and n-dodecane) was also investigated on protease production by the isolate. Protease production by Halobacterium sp. SP1(1) was enhanced by 1.2 folds in presence of n-decane compared to control. Furthermore, the ability of isolate to hydrolyse fish protein was investigated using three different edible fishes (Pomfret, Flat fish and Seer fish) as sole protein source. Pomfret was found to be a good protein source for protease production by the isolate. These results revealed that Halobacterium sp. SP1(1) may have potential for paint-based antifouling coating preparations and fish sauce preparation by virtue of its extracellular protease.

Keywords

ARDRA Fish protein Halobacterium Halophilic archaea Organic solvents Protease Salt pans 

Notes

Acknowledgement

The work was supported by Council of Scientific and Industrial Research (CSIR) grant (90(0038)/04/EMR-II), New Delhi, India. The standard strain Halobacterium salinarum was gifted by Dr. H. M. Sonawat, Tata Institute of Fundamental Research, Mumbai, India. Heartiest thanks to Prof. A. J. Desai, M. S. University of Baroda, India, for providing lab facility and guidance.

Compliance with ethical standards

Conflict of interest

Authors report no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Microbiology and Biotechnology Centre, Faculty of ScienceThe Maharaja Sayajirao University of BarodaVadodaraIndia
  2. 2.Department of BiotechnologyShree M. & N. Virani Science CollegeRajkotIndia

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