Summary
A fungal isolate identified asRhizopus oryzae, produces an extracellular alkaline serine protease. Maximum protease formation was after six days in shake flask culture at two different conditions of pH and temperature optimum (pH 5 at 30°C and pH 10 at 37°C). AgNO3 and Tween 80 increased protease synthesis. The enzyme is stable between pH 3 and pH 11 and has a temperature optimum of 60°C.
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References
Aunstrup, K (1979). Appl. Biochem. Bioengg. 2, 27.
Aunstrup, K (1980), Economic Microbiology, vol 5, Academic Press, London p 50.
Fogarty, W.M., and Kelly, C.T. (1979), Wiseman A (eds) Topics in Enzyme and Fermentation Biotechnology, vol.3., John Wiely & Sons, London p. 45.
Godfrey, T., and Reichelt, J. (1983) eds. Industrial Enzymology, New York, Nature Press.
Kalisz, H. M., (1987), Advances in Biochemical Engg/Biotechnology. A Fiechter vol.36, (Enzyme Study).
Ruttlaff, H. (1981), Stand der Entwicklungstendenzen der mikrobiellen Enzym Produktion. Abh Akad Wiss DDR, Abt Math Naturwiss, Techn. (3N, Mikrob Enzymprod.), p.11.
Sakka, K., Shimada, K., Matsushima, K. (1983), J. Ferment Technol., 63, 480.
Schindler J., Lehmann, R., Pfeiffer H., and Schmid, R. (1983), Enzyme Technology, R.M. Lafferdy eds. p.69, Berlin: Springer-Verlag.
Subramanian, E., Swan, I.D.A., David, R. (1976), Biochem. Biophys. Res. Commun. 68, 875.
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Banerjee, R., Bhattacharyya, B.C. Extracellular alkaline protease of newly isolatedRhizopus oryzae . Biotechnol Lett 14, 301–304 (1992). https://doi.org/10.1007/BF01022328
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DOI: https://doi.org/10.1007/BF01022328