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Analysis of genetic diversity and population structure of confectionery sunflower (Helianthus annuus L.) native to Iran

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Abstract

Genetic diversity within and among 50 populations of confectionery sunflower (Helianthus annuus L.) collected from different geographical areas of Iran was evaluated by using microsatellite and retrotransposon markers. The number of alleles (Na) in SSR loci ranged from 2 to 3 with an average of 2.1. The polymorphic bands in retrotransposon markers ranged from 7 in locus CR-UR1 to 15 in locus CR-816 with a mean value of 11.33. Herarchical clustering of individuals (50 × 5 = 250) by neighbor joining method in DARwin5 software subdivided them into three groups. Using Bayesian method in the software pakage of Structure, the studied individuals were subdivided into two sub-populations. Principal coordinate analysis revelaed that the two first components explaine 7.86 and 6.16% of the total variance, respectively. Analysis of molecular variance revealed a higher level of genetic variation within (70%) than between (30%) populations. High molecular variation among individuals within population possibly is due to high allogamy nature of the sunflower plant. Low genetic variation observed between populations could be considered as a consequence of genetic equilibrium that has occurred over the long period of cultivation of confectionery sunflower in this area as well as seed exchange among regions. The traditional assumption that selecting genotypes of different geographical origin will maximize the diversity available to a breeding project does not hold in confectionery sunflower.

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Authors and Affiliations

  1. Institute of Biotechnology, Urmia University, Urmia, Iran

    Marjan Jannatdoust

  2. Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran

    Reza Darvishzadeh & Roghayyeh Ziaeifard

  3. Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran

    Mohammad Ali Ebrahimi

  4. Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran

    Hamid Hatami Maleki

  5. Department of Agronomy, Payame Noor University, Tehran, Iran

    Esmaeel Gholinezhad

  6. Department of Biology, Faculty of Science, Ilam University, Ilam, Iran

    Ali Asghar Hatamnia

Authors
  1. Marjan Jannatdoust
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  2. Reza Darvishzadeh
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  3. Roghayyeh Ziaeifard
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  4. Mohammad Ali Ebrahimi
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  5. Hamid Hatami Maleki
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  6. Esmaeel Gholinezhad
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  7. Ali Asghar Hatamnia
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Correspondence to Reza Darvishzadeh.

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Jannatdoust, M., Darvishzadeh, R., Ziaeifard, R. et al. Analysis of genetic diversity and population structure of confectionery sunflower (Helianthus annuus L.) native to Iran. J. Crop Sci. Biotechnol. 19, 37–44 (2016). https://doi.org/10.1007/s12892-015-0052-6

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  • DOI: https://doi.org/10.1007/s12892-015-0052-6

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