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Tebuconazole and trifloxystrobin regulate the physiology, antioxidant defense and methylglyoxal detoxification systems in conferring salt stress tolerance in Triticum aestivum L.

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Abstract

Fungicides are widely used for controlling fungi in crop plants. However, their roles in conferring abiotic stress tolerance are still elusive. In this study, the effect of tebuconazole (TEB) and trifloxystrobin (TRI) on wheat seedlings (Triticum aestivum L. cv. Norin 61) was investigated under salt stress. Seedlings were pre-treated for 48 h with fungicide (1.375 µM TEB + 0.5 µM TRI) and then subjected to salt stress (250 mM NaCl) for 5 days. Salt treatment alone resulted in oxidative damage and increased lipid peroxidation as evident by higher malondialdehyde (MDA) and hydrogen peroxide (H2O2) content. Salt stress also decreased the chlorophyll and relative water content and increased the proline (Pro) content. Furthermore, salt stress increased the dehydroascorbate (DHA) and glutathione disulfide (GSSG) content while ascorbate (AsA), the AsA/DHA ratio, reduced glutathione (GSH) and the GSH/GSSG ratio decreased. However, a combined application of TEB and TRI significantly alleviated growth inhibition, photosynthetic pigments and leaf water status improved under salt stress. Application of TEB and TRI also decreased MDA, electrolyte leakage, and H2O2 content by modulating the contents of AsA and GSH, and enzymatic antioxidant activities. In addition, TEB and TRI regulated K+/Na+ homeostasis by improving the K+/Na+ ratio under salt stress. These results suggested that exogenous application of TEB and TRI rendered the wheat seedling more tolerant to salinity stress by controlling ROS and methylglyoxal (MG) production through the regulation of the antioxidant defense and MG detoxification systems.

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Acknowledgements

This research was funded by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. We acknowledge Dennis Murphy, Ehime University, Japan for critical review and English language correction of the manuscript.

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Authors and Affiliations

  1. Laboratory of Plant Stress Responses, Department of Applied Biological Sciences, Faculty of Agriculture, Kagawa University, Miki-Cho, Kita-Gun, Kagawa, 761-0795, Japan

    Sayed Mohammad Mohsin, Md. Shahadat Hossain, M. H. M. Borhannuddin Bhuyan, Khursheda Parvin & Masayuki Fujita

  2. Department of Plant Pathology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Sayed Mohammad Mohsin

  3. Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Mirza Hasanuzzaman

  4. Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Kamrun Nahar

  5. Bangladesh Agricultural Research Institute, Citrus Research Station, Jaintapur, Sylhet, 3156, Bangladesh

    M. H. M. Borhannuddin Bhuyan

  6. Department of Horticulture, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Khursheda Parvin

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Contributions

SMM conceived, designed, and performed the experiment and prepared the manuscript. MH designed the experiment and analyzed the data. KN designed the experiment. MSH, MHMBB, and KP actively participated in executing the experiment. MF conceived, designed, and monitored the experiment.

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Correspondence to Masayuki Fujita.

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Mohsin, S.M., Hasanuzzaman, M., Nahar, K. et al. Tebuconazole and trifloxystrobin regulate the physiology, antioxidant defense and methylglyoxal detoxification systems in conferring salt stress tolerance in Triticum aestivum L.. Physiol Mol Biol Plants 26, 1139–1154 (2020). https://doi.org/10.1007/s12298-020-00810-5

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