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Backbone resonance assignment for the full length tRNA-(N1G37) methyltransferase of Pseudomonas aeruginosa

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Abstract

Bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) plays important roles in translation, making it an important target for the development of new antibacterial compounds. TrmD comprises two domains with the N-terminal domain binding to the S-adenosyl-l-methionine (SAM) cofactor and the C-terminal domain critical for tRNA binding. Bacterial TrmD is functional as a dimer. Here we report the backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa. Most resonances were assigned and the secondary structure for each amino acid was determined according to the assigned backbone resonances. The availability of the assignment will be valuable for exploring molecular interactions of TrmD with ligands, inhibitors and tRNA.

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Acknowledgements

CK appreciates the support from NMRC OF-IRG Grant (NMRC/OFIRG/0051/2017) and A*STAR JCO Grant (1431AFG102/1331A028). This work is also supported by National Research Foundation of Singapore through the Singapore-MIT-Alliance for Research and Technology (SMART) Infectious Disease and Antimicrobial Resistance Interdisciplinary Research Groups. WZ was supported by a SMART Scholar Fellowship. We also thank Prof Ho Sup Yoon and Dr. Hong Ye from Nanyang Technological University for the NMR experiments. The authors appreciate the valuable discussion from the team members at EDDC, A*STAR.

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Author notes

  1. Yan Li

    Present address: Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, Hubei, People’s Republic of China

  2. Yan Li and Wenhe Zhong have contributed equally to this work.

Authors and Affiliations

  1. Experimental Drug Development Centre, 10 Biopolis Road, #05-01, Singapore, 138670, Singapore

    Yan Li, Ann Zhufang Koay, Hui Qi Ng, Jeffrey Hill & CongBao Kang

  2. Infectious Disease and Antimicrobial Resistance Interdisciplinary Research Groups, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, Singapore, 138602, Singapore

    Wenhe Zhong, Qianhui Nah & Peter C. Dedon

  3. NTU Institute of Structural Biology, Nanyang Technological University, Singapore, 636921, Singapore

    Wenhe Zhong, Yee Hwa Wong & Julien Lescar

  4. School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore

    Yee Hwa Wong & Julien Lescar

  5. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA

    Peter C. Dedon

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  1. Yan Li
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  2. Wenhe Zhong
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  7. Jeffrey Hill
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Correspondence to Julien Lescar, Peter C. Dedon or CongBao Kang.

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Li, Y., Zhong, W., Koay, A.Z. et al. Backbone resonance assignment for the full length tRNA-(N1G37) methyltransferase of Pseudomonas aeruginosa. Biomol NMR Assign 13, 327–332 (2019). https://doi.org/10.1007/s12104-019-09900-2

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