Abstract
RNA modification is a post-transcriptional process by which certain nucleotides are altered after their initial incorporation into an RNA chain. Transfer RNAs (tRNAs) is the most heavily modified class of RNA molecules. These modifications expand the chemical and functional diversity of tRNAs and enhance their structural stability. To date, more than 100 modifications have been identified, the majority of which are specific from one domain of life. However, few modifications are extensively present in the three domains of life. Among those, the m1A nucleotide, which consists in the methylation at position 1 of the adenine aromatic ring, is found in tRNAs and ribosomal RNAs. In tRNAs, the m1A modification occurs at position 9, 14, 22, 57 and 58. The enzyme TrmK catalyzes the m1A formation at position 22. Here we report the backbone 1H, 15N and 13C chemical shift assignments of TrmK from Bacillus subtilis obtained by heteronuclear multidimensional NMR spectroscopy as well as its secondary structure in solution as predicted by TALOS+. These assignments of TrmK pave the way for interaction studies with its tRNA substrates.
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Acknowledgments
Financial support from the TGIR-RMN-THC FR3050 CNRS for conducting the research is gratefully acknowledged. We thank Nelly Morellet for helpful discussions and technical advice.
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Dégut, C., Barraud, P., Larue, V. et al. Backbone resonance assignments of the m1A22 tRNA methyltransferase TrmK from Bacillus subtilis . Biomol NMR Assign 10, 253–257 (2016). https://doi.org/10.1007/s12104-016-9680-0
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DOI: https://doi.org/10.1007/s12104-016-9680-0