Abstract
Recent improvement in neuroscience has led to new strategies in neural repair. Hair follicle stem cells are high promising source of accessible, active, and pluripotent adult stem cells. They have high affinity to differentiate to neurons. Aside from using cell–scaffold combinations for implantation, scaffolds can provide a suitable microenvironment for cell proliferation, migration, and differentiation. NT-3 is the most interesting neurotrophic factors being an important regulator of neural survival and differentiation. Since treatment duration in neural repair is very important, this study aims to evaluate the effect of NT-3 and poly-l-lactic acid (PLLA) on differentiation time of bulge stem cells of rat hair follicle to neural-like cells. HFSCs of rat whisker was isolated and cultured on PLLA and differentiated with 10 ng/mL NT-3. Biological features of cultured cells were evaluated with immunocytochemistry and flowcytometry methods by using CD34, nestin, and βІІІ-tubulin markers. For cell viability and morphological assessment, MTT assay and SEM were performed. Our results showed that bulge stem cells of hair follicle can express CD34 and Nestin before differentiation. By using NT-3 during differentiation process, the cells showed positive reaction to βІІІ-tubulin antibody. MTT results demonstrated that PLLA significantly increased cell viability. Finally, HFSCs adhesion was confirmed by SEM results. The results indicate that 10 ng/mL NT-3 and PLLA have significant effect on differentiation time of rat HFSCs to neural cells even in 10 days.
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Acknowledgments
This study was financially supported by grant no. 12444 from Tehran University of Medical Sciences. The authors wish to thank the Anatomy Department of Iran University of Medical Sciences and Dr. Saba Arshi from the Immunology Department of Rasoul Akram Hospital, Iran University of Medical Science.
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An erratum to this article is available at http://dx.doi.org/10.1007/s12031-016-0846-0.
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Ghoroghi, F.M., Hejazian, L.B., Esmaielzade, B. et al. Evaluation of the Effect of NT-3 and Biodegradable Poly-l-lactic Acid Nanofiber Scaffolds on Differentiation of Rat Hair Follicle Stem Cells into Neural Cells In Vitro. J Mol Neurosci 51, 318–327 (2013). https://doi.org/10.1007/s12031-013-0073-x
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DOI: https://doi.org/10.1007/s12031-013-0073-x