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Neuronal differentiation of mesenchymal stem cells by polyvinyl alcohol/Gelatin/crocin and beta-carotene

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Abstract

Background

Nerve tissues are important in coordinating the motions and movements of the body. Nerve tissue repair and regeneration is a slow process that might take a long time and cost a lot of money. As a result, tissue engineering was employed to treat nerve tissue lesions. The aim of this study was to investigate the proliferation of C6 cells and human mesenchymal stem cells derived bone marrow (hBMMSCs) differentiate into neuronal-like cells on the polyvinyl alcohol/gelatin/crocin (PVA/Gel/Cro) nanofiber scaffolds in vitro.

Methods

PVA/Gel scaffolds containing crocin in three concentrations (1%, 3%, and 5%) were prepared by the electrospinning method. The human bone marrow-derived mesenchymal stem cells (hBMSCs) differentiation on the PVA/Gel/Cro 5% that induced by beta-carotene (βC), was analyzed during 10 days. Morphology of differentiated cells on the scaffolds was taken by scanning electron microscope (SEM). The expression of the neural cell markers was studied by quantitative reverse transcription- polymerase chain reaction (qRT-PCR) and immunocytochemistry (ICC).

Results

MTT results of C6 cells culture on the scaffolds showed that proliferation and metabolic activity on PVA/Gel scaffold containing crocin 5% (PVA/Gel/Cro 5%) are significantly more than the other concentrations (P = 0.01). MSC differentiation to nerve-like cells was approved by MAP-2 expression at the mRNA level and NESTIN and MAP-2 at the protein level.

Conclusions

These results suggested that PVA/Gel/Cro 5% and βC could lead to hBMSCs differentiation to neural cells.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Niloofar Asghari, Shiva Irani & Marjan Mohamadali

  2. Burn Research Center, Iran University of Medical Sciences, Tehran, Iran

    Mohamad Pezeshki-Moddaress

  3. Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, Iran

    Mojgan Zandi

Authors
  1. Niloofar Asghari
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  2. Shiva Irani
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  3. Mohamad Pezeshki-Moddaress
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  4. Mojgan Zandi
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  5. Marjan Mohamadali
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Correspondence to Shiva Irani.

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Asghari, N., Irani, S., Pezeshki-Moddaress, M. et al. Neuronal differentiation of mesenchymal stem cells by polyvinyl alcohol/Gelatin/crocin and beta-carotene. Mol Biol Rep 49, 2999–3006 (2022). https://doi.org/10.1007/s11033-022-07123-8

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  • DOI: https://doi.org/10.1007/s11033-022-07123-8

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