Abstract
The stiffness of myocardial tissue changes significantly at birth and during neonatal development, concurrent with significant changes in contractile and electrical maturation of cardiomyocytes. Previous studies by our group have shown that cardiomyocytes generate maximum contractile force when cultured on a substrate with a stiffness approximating native cardiac tissue. However, effects of substrate stiffness on the electrophysiology and ion currents in cardiomyocytes have not been fully characterized. In this study, neonatal rat ventricular myocytes were cultured on the surface of flat polyacrylamide hydrogels with elastic moduli ranging from 1 to 25 kPa. Using whole-cell patch clamping, action potentials and L-type calcium currents were recorded. Cardiomyocytes cultured on hydrogels with a 9 kPa elastic modulus, similar to that of native myocardium, had the longest action potential duration. Additionally, the voltage at maximum calcium flux significantly decreased in cardiomyocytes on hydrogels with an elastic modulus higher than 9 kPa, and the mean inactivation voltage decreased with increasing stiffness. Interestingly, the expression of the L-type calcium channel subunit α gene and channel localization did not change with stiffness. Substrate stiffness significantly affects action potential length and calcium flux in cultured neonatal rat cardiomyocytes in a manner that may be unrelated to calcium channel expression. These results may explain functional differences in cardiomyocytes resulting from changes in the elastic modulus of the extracellular matrix, as observed during embryonic development, in ischemic regions of the heart after myocardial infarction, and during dilated cardiomyopathy.
Similar content being viewed by others
References
Jacot, J. G., Martin, J. C., & Hunt, D. L. (2010). Mechanobiology of cardiomyocyte development. Journal of Biomechanics, 43, 93–98.
Berry, M. F., Engler, A. J., Woo, Y. J., Pirolli, T. J., Bish, L. T., Jayasankar, V., Morine, K. J., Gardner, T. J., Discher, D. E., & Sweeney, H. L. (2006). Mesenchymal stem cell injection after myocardial infarction improves myocardial compliance. American Journal of Physiology-Heart and Circulatory Physiology, 290, H2196–H2203.
Young, J. L., & Engler, A. J. (2011). Hydrogels with time-dependent material properties enhance cardiomyocyte differentiation in vitro. Biomaterials, 32, 1002–1009.
Jacot, J. G., McCulloch, A. D., & Omens, J. H. (2008). Substrate stiffness affects the functional maturation of neonatal rat ventricular myocytes. Biophysical Journal, 95, 3479–3487.
Tallawi, M., Rai, R., Boccaccini, A. R., & Aifantis, K. E. (2015). Effect of substrate mechanics on cardiomyocyte maturation and growth. Tissue Engineering Part B Reviews, 21, 157–165.
Engler, A. J., Carag-Krieger, C., Johnson, C. P., Raab, M., Tang, H. Y., Speicher, D. W., Sanger, J. W., Sanger, J. M., & Discher, D. E. (2008). Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating. Journal of Cell Science, 121, 3794–3802.
Spach, M. S., Heidlage, J. F., Barr, R. C., & Dolber, P. C. (2004). Cell size and communication: role in structural and electrical development and remodeling of the heart. Heart Rhythm, 1, 500–515.
Rodriguez, A. G., Han, S. J., Regnier, M., & Sniadecki, N. J. (2011). Substrate stiffness increases twitch power of neonatal cardiomyocytes in correlation with changes in myofibril structure and intracellular calcium. Biophysics Journal, 101, 2455–2464.
Saygili, E., Rana, O. R., Saygili, E., Reuter, H., Frank, K., Schwinger, R. H., Muller-Ehmsen, J., & Zobel, C. (2007). Losartan prevents stretch-induced electrical remodeling in cultured atrial neonatal myocytes. American Journal of Physiology-Heart and Circulatory Physiology, 292, H2898–H2905.
Rana, O. R., Zobel, C., Saygili, E., Brixius, K., Gramley, F., Schimpf, T., Mischke, K., Frechen, D., Knackstedt, C., Schwinger, R. H., Schauerte, P., & Saygili, E. (2008). A simple device to apply equibiaxial strain to cells cultured on flexible membranes. American Journal of Physiology-Heart and Circulatory Physiology, 294, H532–H540.
Engler, A. J., Griffin, M. A., Sen, S., Bonnemann, C. G., Sweeney, H. L., & Discher, D. E. (2004). Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments. Journal of Cell Biology, 166, 877–887.
Pok, S., Benavides, O. M., Hallal, P., Jacot, J. G. (2014) Use of myocardial matrix in a chitosan-based full-thickness heart patch. Tissue Engineering Part A.
Dimitriadis, E. K., Horkay, F., Maresca, J., Kachar, B., & Chadwick, R. S. (2002). Determination of elastic moduli of thin layers of soft material using the atomic force microscope. Biophysics Journal, 82, 2798–2810.
Boudou, T., Ohayon, J., Picart, C., & Tracqui, P. (2006). An extended relationship for the characterization of Young’s modulus and Poisson’s ratio of tunable polyacrylamide gels. Biorheology, 43, 721–728.
Xi, Y., Wu, G., Ai, T., Cheng, N., Kalisnik, J. M., Sun, J., Abbasi, S., Yang, D., Fan, C., Yuan, X., Wang, S., Elayda, M., Gregoric, I. D., Kantharia, B. K., Lin, S. F., & Cheng, J. (2013). Ionic mechanisms underlying the effects of vasoactive intestinal polypeptide on canine atrial myocardium. Circulation: Arrhythmia and Electrophysiology, 6, 976–983.
Osorio, N., & Delmas, P. (2011). Patch clamp recording from enteric neurons in situ. Nature. Protocols, 6, 15–27.
Raman, I. M., & Bean, B. P. (1997). Resurgent sodium current and action potential formation in dissociated cerebellar Purkinje neurons. Journal of Neuroscience, 17, 4517–4526.
Pelham, Jr., R. J., & Wang, Y. (1997). Cell locomotion and focal adhesions are regulated by substrate flexibility. Proceedings of Naturall Academy Science U S A, 94, 13661–13665.
Calvet, D., Wong, J. Y., & Giasson, S. (2004). Rheological monitoring of polyacrylamide gelation: Importance of cross-link density and temperature. Macromolecules, 37, 7762–7771.
Peyton, S. R., Kim, P. D., Ghajar, C. M., Seliktar, D., & Putnam, A. J. (2008). The effects of matrix stiffness and RhoA on the phenotypic plasticity of smooth muscle cells in a 3-D biosynthetic hydrogel system. Biomaterials, 29, 2597–2607.
Bhana, B., Iyer, R. K., Chen, W. L., Zhao, R., Sider, K. L., Likhitpanichkul, M., Simmons, C. A., & Radisic, M. (2010). Influence of substrate stiffness on the phenotype of heart cells. Biotechnology and Bioengineering, 105, 1148–1160.
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006). Matrix elasticity directs stem cell lineage specification. Cell, 126, 677–689.
Leach, J. B., Brown, X. Q., Jacot, J. G., Dimilla, P. A., & Wong, J. Y. (2007). Neurite outgrowth and branching of PC12 cells on very soft substrates sharply decreases below a threshold of substrate rigidity. Journal of Neural Engineering, 4, 26–34.
Shapira-Schweitzer, K., & Seliktar, D. (2007). Matrix stiffness affects spontaneous contraction of cardiomyocytes cultured within a PEGylated fibrinogen biomaterial. Acta Biomaterials, 3, 33–41.
Janmey, P. A., Winer, J. P., Murray, M. E., & Wen, Q. (2009). The hard life of soft cells. Cell Motility and Cytoskeleton, 66, 597–605.
Beningo, K. A., Dembo, M., Kaverina, I., Small, J. V., & Wang, Y. L. (2001). Nascent focal adhesions are responsible for the generation of strong propulsive forces in migrating fibroblasts. Journal of Cell Biology, 153, 881–888.
Linke, W. A. (2008). Sense and stretchability: the role of titin and titin-associated proteins in myocardial stress-sensing and mechanical dysfunction. Cardiovascular Research, 77, 637–648.
Solon, J., Levental, I., Sengupta, K., Georges, P. C., & Janmey, P. A. (2007). Fibroblast adaptation and stiffness matching to soft elastic substrates. Biophysics Journal, 93, 4453–4461.
Byfield, F. J., Reen, R. K., Shentu, T. P., Levitan, I., & Gooch, K. J. (2009). Endothelial actin and cell stiffness is modulated by substrate stiffness in 2D and 3D. Journal of Biomechanics, 42, 1114–1119.
Azeloglu, E. U., & Costa, K. D. (2010). Cross-bridge cycling gives rise to spatiotemporal heterogeneity of dynamic subcellular mechanics in cardiac myocytes probed with atomic force microscopy. American Journal of Physiology-Heart and Circulatory Physiology, 298, H853–H860.
Deitch, S., Gao, B. Z., & Dean, D. (2012). Effect of matrix on cardiomyocyte viscoelastic properties in 2D culture. Molecular and Cellular Biomechanics, 9, 227–249.
Acknowledgments
Funding for this research was provided by the NIH/NHLBI (1R21HL110330-01 to JGJ) and by Texas Children’s Hospital.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Boothe, S.D., Myers, J.D., Pok, S. et al. The Effect of Substrate Stiffness on Cardiomyocyte Action Potentials. Cell Biochem Biophys 74, 527–535 (2016). https://doi.org/10.1007/s12013-016-0758-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12013-016-0758-1