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Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant

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Abstract

A comparative phenotypic and morpho-histological study was carried out on tissue culture-derived truncated leaf syndrome (TLS) and wild-type oil palm seedlings to investigate their phenotypic and morpho-histological differences. On the basis of the percentage of TLS occurence in a clone, the TLS seedlings were categorized into three groups: severe (70–100%), moderate (40–69%) and mild (<40%). Wild and TLS seedlings differ in terms of growth, vigor, leaf size and shape, root number, volume, length as well as the size of shoot apical meristem (SAM). Differences were also found in fresh weight of leaf, root and SAM of TLS in comparison to wild-type seedlings. Depressed and wavy leaf surface, sunken and distorted stomata and coalesced epidermal cells were observed by scanning electron microscopy in TLS seedlings. The size, shape and number of stomata were also different in the TLS leaf compared to the wild type. Longer epidermal cells, depressed epidermal layer, larger sub-epidermal cells and loosely arranged less mesophyll cells were observed in TLS leaf than in wild type. Undifferentiated vascular bundle was found in TLS leaves where metaxylem and phloem were absent and root tips were impaired. The size and leaf primordial arrangement of SAM were remarkably different in TLS compared to wild-type seedlings suggesting that these alterations might be due to smaller SAM. Therefore, further detailed genetic analysis on TLS SAM is needed for clear understanding of TLS occurrence.

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References

  • Anderson JG, Toohey DW, Brune WH (1991) Free radicals within the Antarctic vortex: the role of CFCs in Antarctic ozone loss. Science 251:39–46

    Article  PubMed  CAS  Google Scholar 

  • Apóstolo NM, Brutti CB, Llorente BE (2005) Leaf anatomy of Cynara scolymus l. In successive micropropagation stages. In vitro Cell Dev Biol Plant 41:307–313

  • Basiron Y (2008) Malaysia’s oil palm-hallmark of sustainable development. Glob Oils Fats Bus 5:1–7

    Google Scholar 

  • Basri MW, Siti Nor Akram Henson IE (2004) Oil palm achievements and potential. In: New directions for a diverse planet. Proceedings of the 4th International Crop Science Congress, 26 Sep–1 Oct 2004, Brisbane, Australia. http://www.cropscienceorg.au/icsc2004/pdf/187_wahidmb.pdf.

  • Ciro AR, Vagner ML (2007) Coffee leaf and stem anatomy under boron deficiency. R Bras Ci Solo 31:477–483

    Article  Google Scholar 

  • Clark SE, Running MP, Meyerowitz EM (1993) CALAVATA1, a regulator of meristem and flower development in Arabidopsis. Development 119:397–418

    PubMed  CAS  Google Scholar 

  • El-Bahr MK, Ali ZA, Saker MM (2004) A comparative anatomical study of date palm vitro plants. Arab J Biotech 7:219–228

    Google Scholar 

  • Estrada-Luna AA, Davies FT Jr, Egilla JN (2001) Physiological changes and growth of micropropagated chile ancho pepper plantlets during acclimatization and post-aclimatization. Plant Cell Tissue Organ Cult 66:17–24

    Article  CAS  Google Scholar 

  • Gillbanks RA (2003) Standard agronomic procedures and practices. In: Fairhurst T, Härdter R (eds) Oil palm: management for large and sustainable yields. Potash and Phosphate Institute (PPI), Potash and Phosphate Institute of Canada (PPIC) and International Potash Institute (IPI), pp 115–151

  • Itoh JI, Hasegawa A, Kitano H, Nagato Y (1998) A recessive heterochronic mutation, plastochorns 1, shortens the plastochorn and elongates the vegetative phase in rice. Plant Cell 10:1511–1521

    Article  PubMed  CAS  Google Scholar 

  • Itoh JI, Kitano H, Matsuoka M, Nagato Y (2000) SHOOT ORGANIZATION genes regulate shoot apical meristem organization and the pattern of leaf primordia initiation in rice. Plant Cell 12:2129–2142

    Article  Google Scholar 

  • Jackson D, Hake S (1999) Control of phyllotaxy in maize by the abphyl1 gene. Development 126:315–329

    PubMed  CAS  Google Scholar 

  • Jain SM (2001) Tissue culture-derived variation in crop improvement. Euphytica 118:153–166

    Article  CAS  Google Scholar 

  • Johansson M, Kronestedt-Robards EC, Robards AW (1992) Rose leaf structure in relation to different stages of micropropagation. Protoplasma 166:165–176

    Article  Google Scholar 

  • Kucera B, Leubner-Metzger G, Wellmann E (2003) Distinct ultraviolet-signaling pathways in bean leaves. DNA damage is associated with β-1, 3-glucanase gene induction, but not with flavonoid formation. Plant Physiol 133:1445–1452

    Article  PubMed  CAS  Google Scholar 

  • Noe N, Bonini L (1996) Leaf anatomy of highbush blueberry grown in vitro and during acclimatization to ex vitro conditions. Biol Plant 38:19–25

    Article  Google Scholar 

  • Obara M, Ikeda K, Itoh JI, Nagato Y (2004) Characterization of leaf lateral symmetry 1 mutant in rice. Breed Sci 54:157–163

    Google Scholar 

  • Oh TJ, Cullis MA, Kunert K, Engelborghs I, Swennen R, Cullis CA (2007) Genomic changes associated with somaclonal variation in banana (Musa spp.). Plant Physiol 129:766–774

    Article  CAS  Google Scholar 

  • Oil world (2007) http//www.soyatech.com

  • Olmos E, Hellin E (1998) Ultrastructural differences of hyperhydric and normal leaves from regenerated carnation plants. Sci Hort 75:91–101

    Google Scholar 

  • Pospišilová J, Tichá I, Kadleček P, Haisel D, Plzáková Š (1999) Acclimatization of micropropagated plants to ex vitro conditions. Biol Plant 42:481–497

    Google Scholar 

  • Quaite FE, Sutherland BM, Sutherland JC (1992) Action spectrum for DNA damage in alfalfa lowers predicted impact of ozone depletion. Nature 358:576–578

    Article  CAS  Google Scholar 

  • Rout GR, Samantaray S, Das P (2000) In vitro manipulation and propagation of medicinal plants. Biotechnol Adv 18:91–120

    Article  PubMed  CAS  Google Scholar 

  • Saker MM, Bekheet Taha HS, Fahmy AS, Moursy HA (2000) Detection of somaclonal variations in tissue culture-derived date palm plants using isozyme analysis and RAPD fingerprints. Biol Plant 43:347–351

    Article  CAS  Google Scholar 

  • Sambanthamurthi R, Singh R, Parveez GKA, Meilina OA, Kushairi A (2009) Opportunities for the oil palm via breeding and biotechnology. In: Jain SM, Priyadarshan PM (eds) Breeding plantation tree crops: tropical species. Springer, New York, pp 377–421

  • Schoeberl MR, Hartmann DL (1991) The dynamics of the stratospheric polar vortex and its relation to springtime ozone depletions. Science 251:46–52

    Article  PubMed  CAS  Google Scholar 

  • Schwendiman J, Pannetier C, Michaux-Ferriere N (1988) Histology of somatic embryogenesis from leaf explants of the oil palm Elaeis guineensis. Ann Bot 62:43–52

    Google Scholar 

  • Sisson WB (1986) Effects of UV-B radiation on photosynthesis. In: Worrest RC, Caldwell MM (eds) Stratospheric ozone reduction, solar ultraviolet radiation and plant life, G8. Springer, New York, pp 161–169

  • Stapleton AE (1992) Ultraviolet radiation and plants: burning questions. Plant Cell 4:1353–1358

    Article  PubMed  Google Scholar 

  • Takada S, Tasaka M (2002) Embryonic shoot apical meristem formation in higher plants. J Plant Res 115:411–417

    Article  PubMed  CAS  Google Scholar 

  • Tan CC, Wong G, Soh AC (1999) Acclimatization and handling of oil palm tissue cultured plantlets for large scale commercial production. In: Paper presented at PORIM Intl. Palm Oil Congress, 1–6 Feb, KL

  • Tan YP, Ho YW, Sharma M (1996) Truncated leaf symptoms (TLS) on clonal seedlings. Int Soc Oil Palm Breed Newslett 12:1–5

    Google Scholar 

  • Teramura AH (1983) Effects of ultraviolet-B on the growth and yield of crop plants. Plant Physiol 58:415–427

    Article  CAS  Google Scholar 

  • Tevini M, Teramura AH (1989) UV-B effects on terrestrial plants. Photochem Photobiol 50:479–487

    Article  CAS  Google Scholar 

  • Thomas J, Vijyan D, Joshi SD, Lopez J, Kumar RR (2006) Genetic integrity of somaclonal variants in tea (Camellia sinensis (L.) O Kuntez) as revealed by inter simple sequence repeats. J Biotechnol 123:149–154

    Article  PubMed  CAS  Google Scholar 

  • Willis LB, Gil GA, Lee HLT, Choi DS, Schoenheit J, Ram RJ, Rha C, Sinskey AJ (2008) Application of spectroscopic methods for the automation of oil palm culture. J Oil Palm Res Special Issue Malays MIT Biotechnol Partn Programme 1:1–13

    Google Scholar 

  • Zobayed SMA, Armstrong J, Armstrong W (2001) Leaf anatomy of in vitro tobacco and cauliflower plantlets as affected by different types of ventilation. Plant Sci 161:537–538

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank the TWOWS (Third World Organization for Women in Sciences) for the postgraduate research fellowship to Habib S.H. and the Ministry of Higher Education, Malaysia for the FRGS grant [(FRGS/FASA -1-2007/(Science Tulen/UPM (No. Ruj 271)] and FELDA Agricultural Services Sdn. Bhd. for providing the oil palm seedlings.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    S. H. Habib, C.-L. Ho & P. Namasivayam

  2. Felda Biotechnology Centre, PT 23417, Lengkuk Technology, Persiaran Technology, Techpark @ Enstek, 71760, Bandar Enstek, Negeri Sembilan, Malaysia

    S. S. R. Syed-Alwee

  3. Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    C.-L. Ho & P. Namasivayam

  4. Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board, No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor, Malaysia

    M. Ong-Abdullah

  5. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    U. R. Sinniah

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  1. S. H. Habib
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  2. S. S. R. Syed-Alwee
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  3. C.-L. Ho
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  4. M. Ong-Abdullah
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  5. U. R. Sinniah
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  6. P. Namasivayam
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Correspondence to P. Namasivayam.

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Communicated by P. Sowinski.

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Habib, S.H., Syed-Alwee, S.S.R., Ho, CL. et al. Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant. Acta Physiol Plant 34, 17–28 (2012). https://doi.org/10.1007/s11738-011-0800-2

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  • DOI: https://doi.org/10.1007/s11738-011-0800-2

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