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Acta Physiologiae Plantarum

, Volume 33, Issue 2, pp 437–442 | Cite as

Effect of temperature on the dose–response curves for auxin-induced elongation growth in maize coleoptile segments

  • Małgorzata Polak
  • Zbigniew Tukaj
  • Waldemar KarczEmail author
Original Paper

Abstract

The dose–response curves for IAA-induced growth in maize coleoptile segments were studied as a function of time and temperature. In addition, the kinetics of growth rate responses at some auxin concentrations and temperatures was also compared. It was found that the dose–response curves for IAA-induced elongation growth were, independently of time and temperature, bell-shaped with an optimal concentration at 10−5 M IAA. The kinetics of IAA-induced growth rate responses depended on IAA concentration and temperature, and could be separated into two phases (biphasic reaction). The first phase (very rapid) was followed by a long lasting one (second phase), which began about 30 min after auxin addition. For coleoptile segments incubated at 30°C, the amplitudes of the first and second phase were significantly higher, when compared with 25°C, at all IAA concentrations studied. However, when coleoptile segments were incubated at 20°C, the elongation growth of coleoptile segments treated with suboptimal IAA concentrations was diminished, mainly as a result of both phases reduction. In conclusion, we propose that the shape of the dose–response curves for IAA-induced growth in maize coleoptile segments is connected with biphasic kinetic of growth rate response.

Keywords

Auxin Coleoptile segments Elongation growth Growth rate Temperature 

Abbreviations

IAA

Indole-3-acetic acid

PM

Plasma membrane

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  • Małgorzata Polak
    • 1
  • Zbigniew Tukaj
    • 2
  • Waldemar Karcz
    • 1
    Email author
  1. 1.Department of Plant Physiology, Faculty of BiologyUniversity of SilesiaKatowicePoland
  2. 2.Department of Plant Physiology, Faculty of BiologyUniversity of GdańskGdyniaPoland

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