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Effect of temperature on the dose–response curves for auxin-induced elongation growth in maize coleoptile segments

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Abstract

The dose–response curves for IAA-induced growth in maize coleoptile segments were studied as a function of time and temperature. In addition, the kinetics of growth rate responses at some auxin concentrations and temperatures was also compared. It was found that the dose–response curves for IAA-induced elongation growth were, independently of time and temperature, bell-shaped with an optimal concentration at 10−5 M IAA. The kinetics of IAA-induced growth rate responses depended on IAA concentration and temperature, and could be separated into two phases (biphasic reaction). The first phase (very rapid) was followed by a long lasting one (second phase), which began about 30 min after auxin addition. For coleoptile segments incubated at 30°C, the amplitudes of the first and second phase were significantly higher, when compared with 25°C, at all IAA concentrations studied. However, when coleoptile segments were incubated at 20°C, the elongation growth of coleoptile segments treated with suboptimal IAA concentrations was diminished, mainly as a result of both phases reduction. In conclusion, we propose that the shape of the dose–response curves for IAA-induced growth in maize coleoptile segments is connected with biphasic kinetic of growth rate response.

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Abbreviations

IAA:

Indole-3-acetic acid

PM:

Plasma membrane

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Authors and Affiliations

  1. Department of Plant Physiology, Faculty of Biology, University of Silesia, Jagiellońska 28, 40032, Katowice, Poland

    Małgorzata Polak & Waldemar Karcz

  2. Department of Plant Physiology, Faculty of Biology, University of Gdańsk, Piłsudskiego 46, 81378, Gdynia, Poland

    Zbigniew Tukaj

Authors
  1. Małgorzata Polak
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  2. Zbigniew Tukaj
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  3. Waldemar Karcz
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Correspondence to Waldemar Karcz.

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Communicated by P. Sowinski.

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Polak, M., Tukaj, Z. & Karcz, W. Effect of temperature on the dose–response curves for auxin-induced elongation growth in maize coleoptile segments. Acta Physiol Plant 33, 437–442 (2011). https://doi.org/10.1007/s11738-010-0563-1

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  • DOI: https://doi.org/10.1007/s11738-010-0563-1

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