Abstract
Catharanthus roseus L. (G) Don. is an important dicotyledonous medicinal plant that produces anticancer compounds, which are used for the treatment of a wide variety of cancers. We have quantified vinblastine (a major dimeric anticancer compound) in various in vitro raised tissues; embryogenic and nonembryogenic calli, three different embryogenic stages (proliferated, matured, and germinating embryo), somatic embryo derived plantlets and in ex vitro grown plantlets by using high performance liquid chromatography. Of the various obtained callus lines and embryogenesis stages, maximum vinblastine content was found in leaf callus and in germinating embryos. The leaves of somatic embryo-derived plantlets contained more vinblastine than did Catharanthus leaves developed ex vitro. The yield of vinblastine was monitored for 30 wk. The production of vinblastine appeared to be age dependent and tissue specific; the finding of our analyses is discussed in detail.
Similar content being viewed by others
References
Bhat M. A.; Ahmad S.; Junaid A.; Mujib A.; Mahmooduzzafar. Salinity stress enhanced production of solasodine in Solanum nigrum L. Chem. Pharm. Bull. 56(1): 17–21; 2008.
Chu I.; Bodnar J. A.; White E. L.; Bowman R. N. Quantification of vincristine and vinblastine in Catharanthus roseus plants by capillary zone electrophoresis. J. Chromatogr. 755: 281–288; 1996.
Datta A.; Srivastava P. S. Variation in vinblastine production by Catharanthus roseus during in vivo and in vitro differentiation. Phytochemistry 46: 135–137; 1997.
De Luca V.; Balsevich J.; Tyler R. T.; Eilert U.; Panchuk B. D.; Kurz W. G. W. Biosynthesis of indole alkaloids: developmental regulation of the biosynthetic pathway from tabersonine to vindoline in Catharanthus roseus. J. Plant Physiol. 125: 147–156; 1986.
Endo T.; Goodbody A.; Vikovic J.; Misawa M. Alkaloid production in root and shoot cultures of Catharanthus roseus. Planta Med. 53: 479–482; 1987.
Favretto D.; Piovan A.; Filippini R.; Caniato R. Monitoring the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow-injection electrospray ionization mass spectrometry. Rapid Comm. Mass Spect. 15: 364–369; 2001.
Junaid A.; Bhatt M. A.; Mujib A.; Sharma M. P. Somatic embryo proliferation, maturation and germination in Catharanthus roseus. Plant Cell Tissue Organ Cult. 84: 325–332; 2006.
Junaid A.; Mujib A.; Fatima S.; Sharma M. P. Culture condition effect somatic embryogenesis in Catharanthus roseus L (G.) Don. Plant Biotechnol. Rep. 2: 179–190; 2008.
Kim S. W.; In D. S.; Choi P. S.; Liu J. R. Plant regeneration from immature zygotic embryo-derived embryogenic calluses and cell suspension cultures of Catharanthus roseus. Plant Cell Tissue Organ Cult. 76: 131–135; 2004.
Kim S. W.; Jung K. H.; Song N. H.; Kwak S. S.; Liu J. R. High frequency plant regeneration from anther-derived cell suspension cultures via somatic embryogenesis in Catharanthus roseus. Plant Cell Rep. 13: 319–322; 1994.
Krueger R. J.; Carew D. P.; Lui J. H. C.; Staba E. J. Initiation, maintenance and alkaloid content of Catharanthus roseus leaf organ cultures. Planta Med. 45: 56–57; 1982.
Kuboyama T.; Yokoshima S.; Tokuyama H.; Fukuyama T. Stereocontrolled total synthesis of (+) vincristine. PNAS 101: 11966–11970; 2004.
Meijer A. H.; Verpoorte R.; Hoge J. H. C. Regulation of enzymes and genes involved in terpenoids indole alkaloid biosynthesis in Catharanthus roseus. J. Plant Res. 3: 145–164; 1993.
Miura Y.; Hirata K.; Kurano K. N.; Miyamoto K.; Chiida K. Formation of vinblastine in multiple shoot culture of Catharanthus roseus. Planta Med. 54: 18–20; 1988.
Moreno P. R. H.; Van der Heijden R.; Verpoorte R. Cell and tissue cultures of Catharanthus roseus; a literature survey II. Updating from 1988–1993. Plant Cell Tissue Organ Cult. 42: 1–25; 1995.
Mujib, A.; Ilah, A.; Gondotra, N.; Abdin, M. Z. In vitro approaches to improve alkaloid yield in Catharanthus roseus. In: “Biotechnology and Genetic Engineering”Vol IV of “Recent Progress in Medicinal Plants” Govil JN, Kumar PA, Singh VK (eds). SciTech Pub, Houston, USA, pp. 415–440; 2003.
Mujib A.; Samaj J. Somatic embryogenesis. Springer Verlag, Berlin Heidelberg, New York, p 357; 2006.
Mukherjee A. K.; Basu S.; Sarkar N.; Ghosh A. C. Advances in cancer therapy with plant based natural products. Curr. Med. Chem. 8: 1467–1486; 2001.
Murashige T.; Skoog F. A revised medium of rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–497; 1962.
Neumann D.; Kraus G.; Heike M.; Groger D. Indole alkaloid formation and storage in cell suspension cultures of C. roseus. Planta Med. 48: 20–23; 1983.
Pasquali G.; Goddijin O. J. M.; De Wall A.; Verpoorte R.; Schilperoort R. A.; Hoge J. H. C. Coordinated regulation of two indole alkaloid biosynthetic genes from Catharanthus roseus by auxin and elicitors. Plant Mol. Biol. 18: 1121–1131; 1992.
Rocha L. K.; Oliveira A. J. B.; Mangolin C. A.; Machado M. F. P. S. Effect of different culture medium components on production of alkaloids in callus tissues of Cereus peruvianus (Cactaceae). Acta Sci. Biol. Sci. 27: 37–41; 2005.
Rommens C. M.; Humara J. M.; Ye Yan H.; Richael C.; Zhang L.; Perry R.; Swords K. Crop improvement through modification of the plant's own genome. Plant Physiol. 135: 421–431; 2004.
Thorpe T. A. In vitro embryogenesis in plants. Kluwer, Dordrecht Netherlands; 1995.
Van der Heijden R.; Jacobs D. T.; Snoeijer W.; Hallard D.; Verpoorte R. The catharanthus alkaloids: pharmacognsosy and biochemistry. Curr. Med. Chem. 11: 607–628; 2004.
Van der Heijden R.; Verpoorte R.; Ten Hoopen H. J. G. Cell and tissue cultures of Catharanthus roseus (L). G. Don: a literature survey. Plant Cell Tissue Organ Cult. 18: 231–280; 1989.
Verpoorte R.; Van der Heijden R.; Schripsema J.; Hoge J. H. C.; Ten Hoopen H. J. G. Plant biotechnology for the production of alkaloids: present status and prospects. J. Nat. Prod. 56: 186–207; 1993.
Walter C. Genetic engineering in conifer forestry: technical and social consideration. In vitro cell. Dev. Biol. Plant 40: 434–441; 2004.
Acknowledgments
The first author is highly grateful to AFMI for getting fellowship during the course of present study.
Author information
Authors and Affiliations
Corresponding author
Additional information
Editor: D. T. Tomes
Rights and permissions
About this article
Cite this article
Aslam, J., Mujib, A., Fatima, Z. et al. Variations in vinblastine production at different stages of somatic embryogenesis, embryo, and field-grown plantlets of Catharanthus roseus L. (G) Don, as revealed by HPLC. In Vitro Cell.Dev.Biol.-Plant 46, 348–353 (2010). https://doi.org/10.1007/s11627-010-9290-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11627-010-9290-y