Abstract
Alkalophilic Bacillus subtilis ASH produced high levels of xylanase using easily available inexpensive agricultural waste residues such as wheat bran, wheat straw, rice husk, sawdust, gram bran, groundnut and maize bran in solid-state fermentation (SSF). Among these, wheat bran was found to be best substrate. Xylanase production was highest after 72 h of incubation at 37 °C and at a substrate to moisture ratio of 1:2 (w/v). The inoculum level of 15% resulted in maximum production of xylanase. The enzyme production was stimulated by the addition of nutrients such as yeast extract, peptone and beef extract. In contrast, addition of glucose and xylose repressed the production of xylanase. The extent of repression by glucose (10%, w/v) was 81% and it was concentration-dependent. Supplementation of the medium with 4% xylose caused 59% repression. Under optimized conditions, xylanase production in SSF (8,964 U of xylanase/g dry wheat bran) was about twofold greater than in submerged fermentation. Thus, B. subtilis produced a very high level of xylanase in SSF using inexpensive agro-residues, a level which is much higher than that reported by any other bacterial isolate. Furthermore, the enzyme was produced at room temperature and with tap water without the addition of any mineral salt in SSF, leading to a marked decrease in the cost of xylanase production, which enhances its industrial potential.
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References
Archana A, Satyanarayana T (1997) Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation. Enzyme Microb Technol 21(7):12–17
Babu KR, Satyanarayana T (1995) α-Amylase production by thermophilic Bacillus coagulans in solid-state fermentation. Process Biochem 30:305–309
Bajpai B, Bhardwaj NK, Bajpai PK, Jauhari MB (1994) The impact of xylanases on bleaching of eucalyptus kraft pulp. J Biotechnol 38:1–6
Ball AS, McCarthy AJ (1989) Saccharification of straw by actinomycete enzymes. J Appl Bacteriol 66:439–444
Battan B, Sharma J, Kuhad RC (2006) High-level xylanase production by alkaliphilic Bacillus pumilus ASH under solid state fermentation. World J Microbiol Biotechnol 22:1281–1287
Beg QK, Bhushan B, Kappor M, Hoondal GS (2000) Enhanced production of a thermostable xylanase from Streptomyces sp. QG-11-3 and its application in biobleaching of eucalyptus craft pulp. Enzyme Microb Technol 27:459–466
Beg QK, Kapoor M, Mahajan L, Hoondal GS (2001) Microbial xylanases and their industrial applications: a review. Appl Microbiol Biotechnol 56:326–338
Chahal PS, Chahal DS, Lee GBB (1996) Production of cellulase in solid-state fermentation with Trichoderma reesi MCG 80 on wheat straw. Appl Biochem Biotechnol 57/58:432–433
Eriksson KEL (1990) Biotechnology in the pulp and the paper industry. Wood Sci Technol 24:79–101
Feniksova RV, Tikhomrova AS, Rakhleeve BE (1960) Conditions for forming amylase and proteinase in surface culture of Bacillus subtilis. Mikrobiologica 29:109–117
Gessesse A, Mamo G (1999) High-level xylanase production by an alkaliphilic Bacillus sp. by using solid-state fermentation. Enzyme Microb Technol 25:68–72
Gilbert HJ, Hazlewood GP (1993) Bacterial cellulases and xylanases. J Gen Microbiol 139:187–194
Haltrich D, Nidetzky B, Kulbe KD, Steiner W, Zupaneie S (1996) Production of fungal xylanases. Bioresour Technol 58:137–161
Heck J, Flores S, Hertzm P, Ayub M (2005) Optimization of cellulase free xylanase activity by Bacillus coagulans BL69 in solid state cultivation. Process Biochem 40:107–112
Hrmova M, Biely P, Vrsanska M, Petrakova E (1984) Induction of cellulose and xylan degrading enzyme complex in the yeast Trichosporon cutaneum. Arch Microbiol 161:371–376
Jain A (1995) Production of xylanase by thermophilic Melanocarpus albomyces IIS 68. Process Biochem 30:705–709
Jecu L (2000) Solid-state fermentation of agricultural wastes for endoglucanase production. Ind Crops Prod 11:1–5
Khandeparkar RDS, Bhosle NB (2006) Isolation, purification and characterization of the xylanase produced by Arthrobacter sp. MTCC 5214 when grown in solid state fermentation. Enzyme Microb Technol 39:732–742
Kuhad RC, Singh A (1993) Lignocellulose biotechnology: current and future prospects. Crit Rev Biotechnol 13:151–172
Liu W, Zhu W, Lu Y, Kong Y, Ma G (1998) Production, partial purification and characterization of xylanase from Trichosporon cutaneum SL409. Process Biochem 33:331–336
Maheswari U, Chandra TS (2000) Production and potential application of a xylanase from a new strain of Streptomyces cuspidosporus. World J Microbiol Biotechnol 16:257–263
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Narahara H, Koyama Y, Yoshida T, Pichangukura S, Ueda R, Taguchi H (1982) Growth and enzyme production in a solid-state culture of Aspergillus oryzae. J Ferment Technol 7:258–265
Puls I, Schuseil I (1993) Chemistry of hemicelluloses: relationship between hemicellulose structure and enzymes required for hydrolysis. Portland Press, London, pp 1–28
Raimbault M, Alazard D (1980) Culture method to study fungal growth in solid fermentation. Eur J Appl Microbiol Biotechnol 9:199–209
Rajaram S, Verma A (1990) Production and characterization of xylanase from Bacillus thermoalkalophilus grown on agricultural wastes. Appl Microbiol Biotechnol 34:141–144
Sindhu I, Chhibber S, Caplash N, Sharma P (2006) Production of cellulase free xylanase from Bacillus megaterium by solid state fermentation for biobleaching of pulp. Curr Microbiol 53:167–172
Srinivasan MD, Rele MV (1999) Microbial xylanases for paper industry. Curr Sci 77:137–142
Srivastava KC (1993) Properties of thermostable hemicellulolytic enzymes from Thermomonospora strain 29 grown in solid state fermentation on coffee processing solid state. Biotechnol Lett 11(3):441–465
Sunna A, Antranikian G (1997) Xylanolytic enzymes from fungi and bacteria. Crit Rev Biotechnol 17:39–67
Thiago LR, Kellaway RC (1982) Botanical composition and extent of lignification affecting digestibility of wheat and oat straw and pastalum hay. Anim Feed Sci Technol 7:71–81
Topkas E, Katapodis P, Kekos D, Macris BJ, Christakopoulos P (2003) Production and partial characterization of xylanase by Sporotrichum thermophile under solid state fermentation. World J Microbiol Biotechnol 19:195–198
Viikari L (1994) Xylanase in bleaching: from an idea to the industry. FEMS Microbiol Rev 13:335–350
Whistler RL, Richards EL (1970) The carbohydrate chemistry and biochemistry. Academic Press Inc., New York, pp 469–477
Wizani W, Esterbauer H, Steiner W, Gomes J (1990) Verfauzer Herstellung exo- and endo-cellulase xylanase. A Patent 1030/90
Xia L, Len P (1999) Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry. Process Biochem 34:909–912
Acknowledgements
The financial assistance received in the form of a research project from the DBT, Ministry of Science and Technology, Govt. of India, New Delhi, is duly acknowledged. Mr. Ashwani Sanghi is grateful to Kurukshetra University, Kurukshetra, India for providing a University Research Scholarship during the course of investigation.
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Sanghi, A., Garg, N., Sharma, J. et al. Optimization of xylanase production using inexpensive agro-residues by alkalophilic Bacillus subtilis ASH in solid-state fermentation. World J Microbiol Biotechnol 24, 633–640 (2008). https://doi.org/10.1007/s11274-007-9521-5
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DOI: https://doi.org/10.1007/s11274-007-9521-5