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Molecular Breeding

, 37:106 | Cite as

Marker-assisted breeding of Chinese elite rice cultivar 9311 for disease resistance to rice blast and bacterial blight and tolerance to submergence

  • Yanchang Luo
  • Tingchen Ma
  • Aifang Zhang
  • Kar Hui Ong
  • Zhixiang Luo
  • Zefu Li
  • Jianbo Yang
  • Zhongchao YinEmail author
Article

Abstract

Rice (Oryza sativa L.) is the staple food crop for more than half of the world’s population. The development of hybrid rice is a practical approach to increase rice production. However, rice production was frequently affected by biotic and abiotic stresses. Rice blast and bacterial blight are two major diseases in rice growing regions. Rice plantation is also frequently affected by short-term submergence or seasonal floods in wet seasons and drought in dry seasons. The utilization of natural disease resistance (R) genes and stress tolerance genes in rice breeding is the most economic and efficient way to combat or adapt to these biotic and abiotic stresses. Rice cultivar 9311 is widely planted rice variety, either as inbred rice or the paternal line of two-line hybrid rice. Here, we report the pyramiding of rice blast R gene Pi9, bacterial blight R genes Xa21 and Xa27, and submergence tolerance gene Sub1A in 9311 genetic background through backcrossing and marker-assisted selection. The improved rice line, designated as 49311, theoretically possesses 99.2% genetic background of 9311. 49311 and its hybrid rice, GZ63S/49311, conferred disease resistance to rice blast and bacterial blight and showed tolerance to submergence for over 18 days without significant loss of viability. 49311 and its hybrids had similar agronomic traits and grain quality to 9311 and the control hybrid rice, respectively. The development of 49311 provides an improved paternal line for two-line hybrid rice production with disease resistance to rice blast and bacterial blight and tolerance to submergence.

Keywords

Rice Marker-assisted selection Gene pyramiding Rice blast Rice bacterial blight Submergence tolerance 

Notes

Acknowledgements

We wish to thank G. Liu for 75-1-127, D. J. Mackill for IR64 (Sub1ASub1A), and Y. Zhou for field trial. This research is sponsored by Temasek Foundation and Temasek Life Sciences Laboratory Innovation Fund. Y. Luo conducted the experiments with the help from T. Ma, A. Zhang, K.Ong, Z. Luo, Z. Li and J. Yang. Y. Luo and Z. Yin designed the experiments and wrote the manuscript.

Supplementary material

11032_2017_695_MOESM1_ESM.pdf (424 kb)
ESM 1 (PDF 424 kb)

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Temasek Life Sciences Laboratory, 1 Research LinkNational University of SingaporeSingaporeRepublic of Singapore
  2. 2.Key Laboratory of Rice Genetics and Breeding, Rice Research InstituteAnhui Academy of Agricultural SciencesHefeiChina
  3. 3.Plant Protection Research InstituteAnhui Academy of Agricultural SciencesHefeiChina
  4. 4.Department of Biological SciencesNational University of SingaporeSingaporeRepublic of Singapore

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