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Journal of Muscle Research & Cell Motility

, Volume 26, Issue 6–8, pp 461–465 | Cite as

Twitchin purified from molluscan catch muscles regulates interactions between actin and myosin filaments at rest in a phosphorylation-dependent manner

  • Yasutaka Tsutsui
  • Maki Yoshio
  • Kazuhiro Oiwa
  • Akira Yamada
Article

Abstract

Twitchin, also called mini-titin, is structurally related to the giant elastic protein connectin/titin, and has been found in not only striated but also smooth muscles of bivalves. Many bivalve smooth muscles such as byssus retractor muscles and the opaque part of adductor muscles are known as catch muscles that can maintain high passive tension with little expenditure of energy after they have actively contracted. Twitchin is phosphorylated when this high-tension state (catch state) ceases. Our recent studies revealed that the catch tension is due to interactions between thick and thin filaments in the presence of MgATP at low free Ca2+ concentrations, which can be visualized in vitro under a light microscope (Yamada et al., 2001 Proc Natl Acad Sci USA 98: 6635–6640). We also found that twitchin is essential for the interactions of the catch state in mussel (Mytilus galloprovincialis) catch muscles. In the presence of twitchin, actin filaments bound to purified myosin filaments when twitchin was dephosphorylated by Ser/Thr protein phosphatase 2B, while they did not when it was phosphorylated by cAMP-dependent protein kinase. In the current study we demonstrate the same essential components of the catch state for another bivalve that exhibits catch, i.e., Japanese oyster (Crassostrea gigas).

Keywords

Mytilus Edulis Dependent Protein Kinase Soluble Protein Fraction Catch State Myosin Heavy Chain Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Yasutaka Tsutsui
    • 1
  • Maki Yoshio
    • 2
  • Kazuhiro Oiwa
    • 1
    • 2
  • Akira Yamada
    • 2
  1. 1.Graduate School of Life ScienceUniversity of HyogoKamigori, Ako-gun, HyogoJapan
  2. 2.Kansai Advanced Research CenterNational Institute of Information and Communications TechnologyKobe, HyogoJapan

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